菌株“花5”产胞外多糖发酵条件优化及其生物活性研究  

Optimisation of fermentation conditions of exopolysaccharides produced by"Hua 5"and their bioactivities

作　　者：李欣霖 赵辉 何雪梅 李彪 孙传齐 马洁 侯怡铃[3] 丁祥 LI Xinlin;ZHAO Hui;HE Xuemei;LI Biao;SUN Chuanqi;MA Jie;HOU Yiling;DING Xiang(Key Laboratory of Nanchong City of Ecological Environment Protection and Pollution Prevention in Jialing River Basin,College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,China;Dazhou Academy of Agricultural Sciences,Dazhou 635002,China;College of Life Sciences,China West Normal University,Nanchong 637009,China)

机构地区：[1]西华师范大学,环境科学与工程学院,嘉陵江流域生态环境保护与污染防治南充市重点实验室,四川南充637009 [2]达州市农业科学研究院,四川达州635002 [3]西华师范大学生命科学学院,四川南充637009

出　　处：《中国酿造》2024年第11期137-143,共7页China Brewing

基　　金：四川省科技厅农业科技成果转化资金项目(2022NZZJ0003);四川省转移支付科技成果转移转化示范项目(22ZYZFSF0009,23ZHSF0082);四川省科技厅应用基础面上项目(2022NSFSC0107)。

摘　　要：该研究以野外采集、分离纯化后的“花5”菌株为研究对象,结合传统形态学观察以及分子生物学技术对菌株进行鉴定,采用单因素及响应面试验优化“花5”菌株产胞外多糖的发酵条件。利用傅里叶红外光谱检测胞外多糖的结构特征,并以细胞活力检测试剂盒检测胞外多糖的体外免疫活性及抗肿瘤活性。结果显示,该菌株被鉴定为花脸香蘑(Lepista sordida),其产胞外多糖的最佳发酵条件为温度25℃,接种量90 mg/100 mL,转速180 r/min,在此培养条件下,胞外多糖产量可达6.01 g/L。红外光谱结果显示,花脸香蘑胞外多糖具有吡喃糖结构。生物活性试验结果显示,花脸香蘑胞外多糖可以显著促进小鼠巨噬细胞RAW264.7增殖(P<0.05),增殖率呈现剂量依赖性增加,当花脸香蘑胞外多糖质量浓度为20μg/mL时,其增殖率达到50.16%;花脸香蘑胞外多糖能够极显著地抑制小鼠胃癌细胞(MFC)增殖(P<0.01),当胞外多糖质量浓度为5μg/mL时,其抑制率达到53.41%。该研究初步验证了花脸香蘑胞外多糖具有一定的免疫调节和抑瘤作用。In this study,"Hua 5"strain collected,isolated and purified in the field was selected as the research object.The strain was identified by traditional morphology and molecular biology.The fermentation conditions of exopolysaccharides produced by"Hua 5"strain were optimized by single factor and response surface tests.The structural characteristics of exopolysaccharides were detected by Fourier infrared spectroscopy,and the in vitro immune activity and anti-tumor activity of exopolysaccharides were detected by cell viability assay kits.The results showed that the strain was identified as Lepista sordida,and the optimal fermentation conditions for the production of exopolysaccharides were temperature 25℃,inoculum 90 mg/100 ml,and rotation speed 180 r/min.Under these conditions,the exopolysaccharides contents could reach 6.01 g/L.The infrared spectrum results showed that L.sordida exopolysaccharides had the pyranose structure.The biological activity tests results showed that L.sordida exopolysaccharides could significantly promote the proliferation of mouse macrophages RAW 264.7(P<0.05),and the proliferation rate showed a dose-dependent increase.When the mass concentration of L.sordida exopolysaccharides was 20μg/ml,the proliferation rate reached 50.16%.The L.sordida exopolysaccharides could significantly inhibit the proliferation of mouse gastric cancer cells(MFC)(P<0.01).When the mass concentration of exopolysaccharides was 5μg/ml,the inhibition rate reached 53.41%.This study preliminarily verified that L.sordida exopolysaccharides had certain immunomodulatory and tumor inhibition effects.

关 键 词：花脸香蘑 胞外多糖 发酵条件优化 响应面分析 生物活性 

分 类 号：Q939.97[生物学—微生物学]