Gene Expression Profile Identifies LncRNA AL355974.3 As a Potential Glioma Biomarker  

作　　者：Hao-tian LU Yu-yang CHEN Yong-jia YU Xing-sheng LIAO Hui LIANG Lun LIANG Pan-lin MO Xin-kai HUANG Shuai DING Chang LIU Da-qin FENG 

机构地区：[1]Department of Neurosurgery,The First Affiliated Hospital,Guangxi Medical University,Nanning,530021,China

出　　处：《Current Medical Science》2024年第5期1047-1057,共11页当代医学科学（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(No.82260554);Natural Science Foundation of Guangxi Province(No.2023GXNSFBA026092 and No.2024GXNSFAA010100);Key Research and development Program of Guangxi Province(No.2023AB22116).

摘　　要：Objective Glioma is a central nervous system tumor arising from glial cells.Despite significant advances in diagnosis and treatment,most patients with high-grade gliomas have a poor prognosis.Many studies have shown that long noncoding RNAs(lncRNAs)may play important roles in the development,progression and treatment of many tumors,including gliomas.Molecularly targeted therapy may be a new direction for the adjuvant treatment of glioma.Therefore,we hope that by studying differentially expressed lncRNAs(DElncRNAs)in glioma,we can discover lncRNAs that can serve as biomarkers for glioma and provide better therapeutic modalities for glioma patients.Methods First,the expression of lncRNAs in 5 normal brain(NB)tissues and 10 glioma tissues was examined by RNA sequencing(RNA-seq).Next,we performed Kaplan-Meier analysis of data from The Cancer Genome Atlas(TCGA)database to assess the prognostic value of these variables.Finally,functional analysis of the DElncRNAs was performed by means of Gene Ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Results RNA sequencing analysis revealed 85 upregulated miRNAs and 71 downregulated lncRNAs in low-grade glioma(LGG)and 50 upregulated lncRNAs and 70 downregulated lncRNAs in glioblastoma(GBM).Among them,AL355974.3 was the most upregulated lncRNA.LINC00632 was the most downregulated lncRNA.Second,LGG patients with higher AL355974.3 expression had worse overall survival according to Kaplan-Meier analysis of the TCGA database.Finally,bioinformatics analysis revealed that the target genes of these DElncRNAs were enriched in various biological processes and signaling pathways,such as cell metabolic and developmental processes.Conclusion Our findings provide evidence that AL355974.3 may be a new biomarker for glioma.

关 键 词：GLIOMA long noncoding RNAs BIOMARKER RNA sequencing 

分 类 号：R739.4[医药卫生—肿瘤]