基于聚葡萄糖、甜菊糖苷中蛋白酶活力检测方法的研究  

Study on the Method of Detecting Protease Activity in Polyglucose and Stevia Glycosides

作　　者：张琳 于敏 刘彦明 张亚华 陈娟 逯刚 ZHANG Lin;YU Min;LIU Yanming;ZHANG Yahua;CHEN Juan;LU Gang(Inner Mongolia Mengniu High-Tech Dairy,Co.,Ltd.,Hohhot Inner Mongolia 011500;Inner Mongolia Mengniu Dairy(Group)Co.,Ltd.,Hohhot Inner Mongolia 011500)

机构地区：[1]内蒙古蒙牛高科乳业有限公司,内蒙古呼和浩特011500 [2]内蒙古蒙牛乳业(集团)股份有限公司,内蒙古呼和浩特011500

出　　处：《中国乳业》2024年第11期149-156,共8页China Dairy

基　　金：国家重点研发计划项目(2022YFD1301005)。

摘　　要：[目的]建立聚葡萄糖、甜菊糖苷中蛋白酶活力检测方法。[方法]通过单因素试验、正交试验优化蛋白酶活力的检测条件,考察回收率试验方法准确度。[结果]聚葡萄糖、甜菊糖苷中蛋白酶活力最佳检测条件:硼酸缓冲溶液溶解样品,待测样品取样量5 g,反应时间20 min,反应温度40℃,pH值10.5,滤液静置时间20 min。回收率试验结果表明,方法准确度64%~102%,相对标准偏差<1.3%。[结论]曲线相关性、准确度等验证,该方法适用聚葡萄糖、甜菊糖苷中蛋白酶含量检测。[Objective]The detection method of protease activity in polyglucose and stevioside glycosides was established.[Method]Single factor experiment and orthogonal experiment were used to optimize the assay conditions of protease activity,and the accuracy of the recovery method was investigated.[Result]The optimal conditions for the detection of protease activity in polyglucose and stevioside glycosides were to dissolve samples with boric acid buffer solution,sample size to be tested was 5 g,reaction time was 20 min,reaction temperature was 40℃,pH value was 10.5,and filtrate standing time was 20 min.The experimental results showed that the accuracy of the method was 64%~102%and the relative standard deviation was less than 1.3%.[Conclusion]This method was suitable for the detection of protease content in stevioside polyglucose after comprehensive verification of curve correlation and accuracy.

关 键 词：聚葡萄糖 甜菊糖苷 蛋白酶活力 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]