1株多重耐药副溶血弧菌噬菌体vB_VpP_3的生物学特性及全基因组分析  

Biological Characteristics and Whole Genome Analysis of vB_VpP_3,a Bacteriophage against Multi-drug Resistant Vibrio parahaemolyticus

作　　者：刘青青 张明 杨雯静 刘可 李凡 李学鹏 励建荣 张德福 LIU Qingqing;ZHANG Ming;YANG Wenjing;LIU Ke;LI Fan;LI Xuepeng;LI Jianrong;ZHANG Defu(Institute of Ocean,National and Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Food Safety Key Lab of Liaoning Province,College of Food Science and Engineering,Bohai University,Jinzhou 121013,China)

机构地区：[1]渤海大学食品科学与工程学院,渤海大学海洋研究院,生鲜农产品贮藏加工及安全控制国家地方联合工程研究中心,辽宁省食品安全重点实验室,辽宁锦州121013

出　　处：《食品科学》2024年第24期108-116,共9页Food Science

基　　金：渤海大学海洋研究院开放课题项目(BDHYYJY2024003)。

摘　　要：目的:分离对多重耐药副溶血性弧菌具有较强裂解能力的噬菌体。方法:以副溶血性弧菌为宿主菌,从海产品中分离、纯化噬菌体,对分离到的噬菌体进行生物学特性和全基因组分析。结果:从螃蟹中分离到1株噬菌体,命名为vB_VpP_3。该噬菌体裂解谱较窄,宿主特异性强,其最佳感染复数为0.1,吸附率最高为93%。一步生长曲线显示,该噬菌体的潜伏期为15 min,平均裂解量为110 PFU,且对环境有较强的耐受性。噬菌体vB_VpP_3头部为正二十面体,直径约60 nm,有长约20 nm的不可收缩尾部,属于有尾噬菌体目(Caudovirales)的短尾噬菌体科(Podoviridae)。其基因组为双链线性DNA,全长42 459 bp,总GC含量46.87%,未携带毒力及耐药基因。结论:噬菌体vB_VpP_3具有较高的安全性,可以用于多重耐药副溶血性弧菌的生物防治。Objective:To isolate bacteriophages with strong lytic activity against multi-drug resistant Vibrio parahaemolyticus.Methods:Using V.parahaemolyticus as host bacteria,bacteriophages were isolated and purified from seafood,and their biological characteristics and whole genome were analyzed.Results:Phage vB_VpP_3,which was isolated from crab,had a narrow host range and high host specificity with an optimal multiplicity of infection 0.1 and a maximum adsorption rate of 93%.The one-step growth curve showed that the latent period of the phage was 15 min,the average burst size was 110 PFU,and it was highly tolerant to the environment.Phage vB_VpP_3 belonged to the order Caudovirales of the family Podoviridae with a regular icosahedral head about 60 nm in diameter and a non-contractile tail about 20 nm in length.Its genome was double-stranded linear DNA with a total length of 42459 bp and a total GC content of 46.87%without any virulence or drug resistance genes.Conclusion:Bacteriophage vB_VpP_3 was highly safe and could be used for biological control of multi-drug resistant V.parahaemolyticus.

关 键 词：副溶血性弧菌 噬菌体 生物防治 生物学特性 基因组学分析 

分 类 号：TS201.3[轻工技术与工程—食品科学]