嗜果聚糖Lactiplantibacillus plantarum 19M03的筛选及全基因组测序分析  

Screening and Whole-Genome Sequencing Analysis of Fructofuranose-Metabolizing Lactiplantibacillus plantarum 19M03

作　　者：孙大庆 齐贺 邸子清 葛相麟 洪青平 杜新蕊 姚禹西 李洪飞 SUN Daqing;QI He;DI Ziqing;GE Xianglin;HONG Qingping;DU Xinrui;YAO Yuxi;LI Hongfei(National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural University,Daqing 163319,China;College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Key Laboratory of Agro-products Processing and Quality Safety of Heilongjiang Province,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆163319 [2]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [3]黑龙江省农产品加工与质量安全重点实验室,黑龙江大庆163319

出　　处：《食品科学》2024年第23期113-122,共10页Food Science

基　　金：黑龙江八一农垦大学引进人才科研启动计划项目(XDB202009);黑龙江八一农垦大学三横三纵支持计划项目(ZRCPY202005);黑龙江八一农垦大学研究生创新科研项目(YJSCX2022-Y53);黑龙江八一农垦大学引进人才科研启动基金项目(XDB202404);黑龙江八一农垦大学“三纵”科研支持计划基础培育项目(ZRCPY202228)。

摘　　要：为探究乳酸菌果聚糖代谢机制和开发低果聚糖发酵食品,本研究对287株酸面团来源的乳酸菌进行果聚糖利用能力筛选,获得1株嗜果聚糖乳酸菌19M03,之后利用高通量测序和生物信息学技术对该菌株进行全基因组测序和果聚糖代谢机制分析。测序结果表明,菌株19M03基因组含有1个染色体和9个质粒,基因组全长3246316 bp,GC相对含量44.53%,包含3084个编码基因、66个tRNA和16个rRNA。系统发育关系分析表明,分类学上菌株19M03属于植物乳植杆菌。基因功能注释结果表明,在同源蛋白簇、基因本体论、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)和碳水化合物活性酶数据库中,19M03共编码357个碳水化合物代谢相关基因和351个碳水化合物转运相关基因,其中与果聚糖代谢和转运相关的基因均有12个。根据这些基因在KEGG数据库中的代谢关系,提出19M03果聚糖代谢模型。该模型解析了果聚糖水解过程及其催化反应的细胞定位(既可胞内,又可胞外)、细胞膜中运输低聚果糖和果糖的特异转运蛋白(IIABC^(FOS)、IIABCD^(fru)、IIABC^(fru))、细胞内果糖分解代谢途径(n=4)和果糖合成代谢途径(n=1)及其所有相关酶的编码基因。此外,耐药和毒力基因分析证明19M03具有良好的安全性。总之,本研究筛选获得了1株嗜果聚糖乳酸菌19M03,并在全基因组水平揭示了19M03果聚糖代谢机制,这些结果可为阐明乳酸菌果聚糖代谢机制提供重要的理论参考,为今后低果聚糖功能食品的开发和应用提供优质的菌种资源。This study was conducted to investigate the mechanism of fructan metabolism in lactic acid bacteria in order to develop low-fructan fermented foods.One strain capable of utilizing fructan,19M03,was selected from 287 lactic acid bacteria strains isolated from sourdough.Then,the strain was subjected to whole-genome sequencing and its fructan metabolism mechanism was analyzed by using high-throughput sequencing and bioinformatic techniques.The sequencing results showed that the genome of strain 19M03 contained one chromosome and nine plasmids,with a total length of 3246316 bp,GC content of 44.53%,3084 coding genes,66 tRNAs and 16 rRNAs.The phylogenetic relationship analysis showed that strain 19M03 belonged to Lactiplantibacillus plantarum.The results of gene function annotation showed that its genome sequence contained a total of 357 genes encoding carbohydrate-active enzymes(CAZymes)and 351 genes encoding carbohydrate transport proteins in the Clusters of Orthologous Groups of proteins(COGs),Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and CAZy databases,as well as 12 genes related to fructan metabolism and transport,respectively.Based on the metabolic relationship of these genes in the KEGG database,a fructan metabolism model was proposed for 19M03.The model resolved the cellular localization of the fructan hydrolysis process and related catalytic reactions(both intracellular and extracellular),the specific transporters of fructooligosaccharide and fructose in the cell membrane(IIABC^(FOS),IIABCD^(fru)and IIABC^(fru)),the intracellular pathways of fructose catabolism(n=4)and fructose anabolism(n=1),and the genes encoding all related enzymes.In addition,resistance and virulence gene analyses demonstrated that 19M03 had a good safety profile.In conclusion,these results provide an important theoretical basis for elucidating the fructose metabolism mechanism of lactic acid bacteria so as to provide high-quality strain resources for the development and application of low-fructofuran functional foods

关 键 词：果聚糖代谢 全基因组测序 代谢机制 

分 类 号：TS201.3[轻工技术与工程—食品科学]