虾青素对慢性睡眠剥夺小鼠免疫功能及心肌损伤的影响  

Effect of Astaxanthin on Immune Function and Myocardial Injury in Mice with Chronic Sleep Deprivation

作　　者：冯碧芸 李玲燕 李明 张嘉媛 戚欢阳 陈娉娉 许光辉 FENG Biyun;LI Lingyan;LI Ming;ZHANG Jiayuan;QI Huanyang;CHEN Pingping;XU Guanghui(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Xiamen Key Laboratory of Natural Medicine Research and Development,Xiamen Health and Medical Big Data Center(Xiamen Medicine Research Institute),Xiamen 361008,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]厦门市健康医疗大数据中心(厦门市医药研究所),厦门市天然药物研究与开发重点实验室,福建厦门361008

出　　处：《食品科学》2024年第23期150-158,共9页Food Science

基　　金：厦门市扶持中医药发展专项(XWZY-2023-0605);厦门市海洋与渔业发展专项资金青年创新项目(23YYZP013QCA11);厦门市科技计划项目(3502Z20226034,3502Z20224004);厦门市自然科学基金青年项目(3502720227156);福建省卫生健康科技计划项目(2020QN01010034)。

摘　　要：目的:探讨虾青素对慢性睡眠剥夺(chronic sleep deprivation,CSD)小鼠免疫功能及心肌损伤的影响。方法:运用改良多平台水环境法连续2周建立CSD小鼠模型,同时灌胃不同剂量的虾青素干预,通过脏器系数、碳廓清实验和酶联免疫吸附试剂盒检测虾青素对CSD小鼠免疫功能及心肌损伤的作用,并且利用网络药理学技术对虾青素改善CSD小鼠免疫功能及心肌损伤的作用靶点、生物过程及相关通路进行分析。结果:与模型组比较,虾青素能显著增加治疗组小鼠的体质量、吞噬指数和廓清指数,降低其心、脾、胰腺的脏器系数,同时,各给药组小鼠血清肌酸激酶、超氧化物歧化酶、肿瘤坏死因子-α、谷胱甘肽过氧化物酶、白细胞介素-1β、白细胞介素-6、一氧化氮合酶、γ-谷氨酰转肽酶含量显著升高,丙二醛水平显著降低。结论:虾青素对CSD导致的免疫功能低下和心肌损伤有显著地改善作用,可能与其调控神经营养因子、Toll样受体、核因子-κB、T细胞受体、细胞凋亡信号通路,抑制炎症反应有关。Objective:To explore the effect of astaxanthin on immune function and myocardial injury in mice with chronic sleep deprivation(CSD).Methods:A modified multi-platform water environment method was used to establish an CSD mouse model for two weeks.At the same time,mice were intragastrically administered with astaxanthin at different doses.Immune function and myocardial injury were evaluated by measuring organ coefficients as well as using the carbon clearance test and commercial enzyme-linked immunosorbent assay(ELISA)kits.The targets of astaxanthin and related biological processes and pathways were analyzed by network pharmacology.Results:Compared with the model group,body mass,phagocytic index and clearance index significantly increased in the treatment groups,and heart,spleen and pancreas coefficients decreased.Moreover,the levels of serum creatine kinase(CK),superoxide dismutase(SOD),tumor necrosis factor-α(TNF-α),glutathione peroxidase(GSH-Px),interleukin(IL)-1β,IL-6,nitric oxide synthase(NOS)andγ-glutamyl transpeptidase(γ-GT)rose markedly in each treatment group,and the level of malondialdehyde(MDA)significantly declined.Conclusion:Astaxanthin greatly ameliorates immune dysfunction and myocardial injury caused by CSD,perhaps by regulating the neurotrophic factors,Toll-like receptor,nuclear factor-κB(NF-κB),T cell receptor and apoptosis signaling pathways and inhibiting inflammatory response.

关 键 词：虾青素 慢性睡眠剥夺 免疫功能 心肌损伤 网络药理学 

分 类 号：R151.2[医药卫生—营养与食品卫生学]