基于Plackett-Burman与Box-Behnken法优化桑叶降胆固醇肽的提取工艺  

Optimization of extraction process of cholesterol-lowering peptides from mulberry leaves based on Plackett-Burman and Box-Behnken methods

作　　者：段涵怡 郭美霞 谭军 董悦君 赵佳迪 杨雪 DUAN Han-yi;GUO Mei-xia;TAN Jun;DONG Yue-jun;ZHAO Jia-di;YANG Xue(Chinese Medicine Research Institute,Chengde Medical University,Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development,Chengde 067000,Hebei,China;School of Basic Medical Sciences,Chengde Medical University,Chengde 067000,Hebei,China)

机构地区：[1]承德医学院中药研究所,河北省中药研究与开发重点实验室,河北承德067000 [2]承德医学院基础医学院,河北承德067000

出　　处：《粮食与油脂》2024年第12期103-108,147,共7页Cereals & Oils

基　　金：河北省高等学校科学技术研究青年拔尖人才计划项目(BJK2023035);河北省中医药类科学研究课题计划项目(2025088)。

摘　　要：以桑叶为原料,采用碱性蛋白酶酶解制备桑叶降胆固醇肽。以多肽质量浓度及胆固醇抑制率为指标,在单因素试验基础上,采用Plackett-Burman与Box-Behnken法优化桑叶降胆固醇肽的酶解工艺。结果表明:最佳酶解工艺为桑叶蛋白质量浓度9.00g/L、加酶量8.0%(以桑叶蛋白质量计)、酶解时间97min、酶解pH7.0、酶解温度48℃。在此条件下,桑叶多肽质量浓度为0.262 mg/mL,胆固醇抑制率为33.27%。Cholesterol-lowering peptides extracted from mulberry leaf were prepared by enzymolysis with alkaline protease.Using the peptide mass concentration and cholesterol inhibition rate as indicators,and the enzymatic hydrolysis process was optimized by Plackett-Burman and Box-Behnken methods based on single factor experiments.The results showed that the optimal enzymatic hydrolysis process conditions were mulberry leaf protein mass concentration 9.00 g/L,enzyme dosage 8.0%(based on the mass of mulberry leaf protein),enzymatic hydrolysis time 97 min,enzymatic hydrolysis pH 7.0,and enzymatic hydrolysis temperature 48 C.Under these conditions,the mass concentration of the mulberry leaf pep-tide was 0.262 mg/mL,and the inhibition rate of cholesterol was 33.27%.

关 键 词：桑叶蛋白 碱性蛋白酶 酶解工艺 降胆固醇肽 

分 类 号：TS201.1[轻工技术与工程—食品科学]