天津市市售熟食致病菌分离鉴定、耐药及毒力特征分析  

Analysis of contamination risk,drug resistance and virulence characteristics of cooked food pathogens in Tianjin

作　　者：郑宝璐 李闻[2] 张维[2] 于爱萍 鹿晓慧 刘广文[2] 李晓燕[2] 刘运德[1] Zheng Baolu;Li Wen;Zhang Wei;Yu Aiping;Lu Xiaohui;Liu Guangwen;Li Xiaoyan;LiuYunde(School of Medical Laboratory,Tianjin Medical University,Tianjin 300203,China;Department of Pathogenic Microbiology,Tianjin Center for Disease Control and Prevention,Tianjin Key Laboratory of Pathogenic Microbiology of Infectious Disease,Tianjin 300011,China)

机构地区：[1]天津医科大学医学检验学院,天津300203 [2]天津市疾病预防控制中心微生物检验检测所,天津市传染病病原微生物重点实验室,天津300011

出　　处：《中华微生物学和免疫学杂志》2024年第11期943-950,共8页Chinese Journal of Microbiology and Immunology

基　　金：天津市卫生健康委重点项目(TJWJ2022ZD010)。

摘　　要：目的了解天津市市售熟食致病菌污染风险、耐药及毒力特征。方法对2019—2021年天津市市售熟食进行致病菌分离鉴定,对分离株进行药敏试验、全基因组测序,并对数据进行多位点序列分型、耐药基因及表型、毒力基因分析。结果从采集的245份市售熟食样本中分离出10株致病菌,7株沙门菌ST型别为ST40、ST198、ST14、ST13以及ST155;2株副溶血性弧菌分别为ST647型、ST2622型;1株阪崎克罗诺杆菌为ST8型。6株分离株均为耐药株,其中3株分离株为多重耐药株(multidrug-resistaret,MDR)。10株分离株共预测出63个耐药基因,2株沙门菌为氨苄西林-氯霉素-链霉素-磺胺甲噁唑-四环素耐药型(MDR-ACSSuT),2株沙门菌均携带gyrA双突变(S83F,D87N)、parC突变(S80I)。副溶血性弧菌主要携带blaCARB及tet(35)基因,阪崎克罗诺杆菌仅携带1个耐药基因blaCSA-1。10株分离株共预测出163个毒力基因。结论本研究中多次在鹌鹑蛋中检出致病菌,警示除鸡蛋外的其他蛋类也要格外关注污染风险。本研究中的分离株均为流行ST型,耐药现象普遍,MDR-ACSSuT株应引起重视,gyrA双突变(S83F、D87N),parC突变(S80I)在喹诺酮类药物耐药性发展中的关键作用不容忽视。T3SS(Ⅲ型分泌系统)、T6SS(Ⅵ型分泌系统)为沙门菌、副溶血性弧菌重要毒力基因簇。Objective To understand the contamination risk,drug resistance and virulence characteristics of cooked food pathogens in Tianjin.Methods Pathogenic bacteria were isolated and identified in cooked food sold in Tianjin from 2019 to 2021,drug susceptibility test and whole genome sequencing were conducted for the isolates,and multilocus sequence typing,drug resistance gene,phenotype and virulence gene were analyzed for the data.Results Ten pathogenic bacteria were isolated from 245 cooked food samples collected.The ST types of 7 Salmonella strains were ST40,ST198,ST14,ST13 and ST155.The two strains of Vibrio parahaemolyticus were ST647 and ST2622 respectively.One strain of Cronobacter sakazakii was ST8 type.All the 6 isolates were drug-resistant,and 3 of them were multidrug-resistant(MDR).A total of 63 drug-resistant genes were predicted in 10 isolates.Two Salmonella strains were ampicillin-chloramphenicol-streptomycin-sulfamethoxazole-tetracycline resistant(MDRACSSuT),and both Salmonella strains carried gyrA double mutations(S83F,D87N)and parC mutations(S80I).Vibrio parahaemolyticus mainly carries blaCARB and tet(35)genes,while Cronobacter sakazakii carries only one resistance gene blaCSA-1.A total of 163 virulence genes were predicted from 10 isolates.Conclusions In this study,pathogenic bacteria are repeatedly detected in quail eggs,warning that other eggs besides chicken eggs should also pay special attention to the risk of contamination.The isolates in this study are all of the prevalent ST type with widespread drug resistance,and MDR-ACSSuT strains should be paid attention to.The key role of gyrA double mutation(S83F,D87N)and parC mutation(S80I)in the development of quinolone resistance should not be ignored.T3SS(typeⅢsecretory system)and T6SS(typeⅥsecretory system)are important virulence gene clusters of Salmonella and Vibrio parahemolyticus.

关 键 词：熟食 全基因组测序 GYRA PARC 耐药 毒力 

分 类 号：R155.5[医药卫生—营养与食品卫生学]