吡喃衍生物传感明胶双通道指示鱼肉新鲜度  

Pyran Derivative Sensing Gelatin Dual-Channel Indication of Fish Freshness

作　　者：钟克利[1] 刘爽[1] 姚远[1] 汤立军[1] 李学鹏[1] 孟玉琼 马睿 励建荣[1] Zhong Keli;Liu Shuang;Yao Yuan;Tang Lijun;Li Xuepeng;Meng Yuqiong;Ma Rui;Li Jianrong(College of Chemistry and Materials Engineering,College of Food Science and Engineering,National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Bohai University,Jinzhou 121013,Liaoning;College of Ecological and Environmental Engineering,Qinghai University,Xining 810000)

机构地区：[1]渤海大学化学与材料工程学院、食品科学与工程学院、生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]青海大学生态环境工程学院,西宁810000

出　　处：《中国食品学报》2024年第10期418-424,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(22278038);辽宁省教育厅科研项目(LJKMZ20221480)。

摘　　要：以苯并吡喃为荧光团,通过引入吸电子基三氰基呋喃延长共轭体系,设计合成了胺类敏感型荧光探针CHM。该探针在EtOH/H2O(5/5,V/V)体系中对11种胺有荧光增强响应,具有较大的斯托克斯位移(120 nm),较低的检测限(腐胺,1.3μmol/L),并可在活细胞中对腐胺荧光成像。将CHM负载到明胶上,制备的传感明胶CHMGL对挥发性胺的颜色变化明显,可通过比色和荧光双通道对三文鱼鱼肉新鲜度进行实时监测,从而准确判断鱼肉的鲜度。In this paper,an amine-sensitive fluorescent probe CHM was designed and synthesized using benzopyran as a fluorophore by introducing an electron-withdrawing group tricyanofuran to extended conjugated system.The probe can recognize 11 amines with an off-on fluorescence response,a large Stokes shift(120 nm),a low detection limit(putrescine,1.3μmol/L)in EtOH/H2O(5/5,V/V)solution,and can imaging for putrescine in living cells.Probe CHM is loaded onto the gelatin to obtain sensing gelatin CHMGL.CHMGL shows significant color changes to volatile amines,which can be used to monitor the freshness of salmon fish in real time through colorimetric and fluorescence dual-channels,and can accurately judge the freshness of fish.

关 键 词：荧光探针 传感明胶 挥发性胺 三文鱼 新鲜度 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]