荧光定量PCR法检测肉制品中小肠结肠炎耶尔森菌  

Detection of Yersinia enterocolitica in Meat Product by Real-Time PCR

作　　者：于明明[1] 孙广雷 汪忆梦 姜丽丹 沈万恒 YU Mingming;SUN Guanglei;WANG Yimeng;JIANG Lidan;SHEN Wanheng(Weifang Center for Disease Control and Prevention,Weifang 261061,China;Kuiwen Center for Disease Control and Prevention,Weifang 261041,China;Yidu Central Hospital,Weifang 262500,China)

机构地区：[1]潍坊市疾病预防控制中心,山东潍坊261061 [2]潍坊市奎文区疾病预防控制中心,山东潍坊261041 [3]潍坊市益都中心医院,山东潍坊262500

出　　处：《食品安全导刊》2024年第35期79-82,共4页China Food Safety Magazine

基　　金：潍坊市科技局科学技术发展计划“潍坊市生肉食品小肠结肠炎耶尔森菌风险评估”(2023YX089)。

摘　　要：目的:进一步提高肉制品中小肠结肠炎耶尔森菌的检出效率。方法:按照《食品安全国家标准食品微生物学检验小肠结肠炎耶尔森氏菌检验》(GB 4789.8—2016)进行样品处理、增菌,采用荧光定量聚合酶链式反应(Polymerase Chain Reaction,PCR)方法对增菌液进行初筛,初筛阳性的样本进行菌株分离,挑取选择性平板上典型菌落进行PCR鉴定及生化验证。结果:荧光定量PCR方法能够检出肉制品中低污染水平(1~10 CFU·mL^(-1))的小肠结肠炎耶尔森菌;352份肉制品中共检出小肠结肠炎耶尔森菌阳性样本34份,总检出率为9.66%,其中禽肉检出率为8.94%(27/302),猪肉检出率为14.0%(7/50)。结论:肉制品中小肠结肠炎耶尔森菌污染率较高,采用荧光定量PCR结合传统生化分离法能提高肉制品中小肠结肠炎耶尔森菌检测效率,减少漏检。Objective:To further enhance the detection efficiency of Yersinia enterocolitica in meat products.Method:Sample processing and bacteria enriching were according to the national food safety standard GB 4789.8—2016,and the bacteria enrichment broth was firstly detected by real-time polymerase chain reaction,then Yersinia enterocolitica strains were isolated from the positive samples and screened by real-time PCR again,finally the positive strains were confirmed by biochemical identification.Result:Real-time PCR method can detect Yersinia enterocolitica with low contamination level(1~10 CFU·mL^(-1))in meat products.34 Yersinia enterocolitica positive samples were detected from 352 meat products,and the total detection rate was 9.66%,the detection rate of poultry meat was 8.94%(27/302)and 14.0%(7/50)for pork.Conclusion:There was relatively high contamination rate of Yersinia enterocolitica in meat products.The method of real-time PCR combined with biochemical identification can improve the detection efficiency and reduce missed detection.

关 键 词：小肠结肠炎耶尔森菌 肉制品 实时荧光聚合酶链式反应 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程] O657.3[轻工技术与工程—食品科学与工程]