Co-administration of Antarctic krill peptide EEEFDATR and calcium shows superior osteogenetic activity  

作　　者：Yao Liu Songyi Lin Shengjie Hu Di Wang Hui Yao Na Sun 

机构地区：[1]National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,Dalian,116034,PR China [2]Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian,116034,PR China

出　　处：《Food Bioscience》2022年第4期74-80,共7页食品生物科学（英文）

基　　金：supported by the LiaoNing Revitalization Talents Program of China(XLYC2007078);the Program for Innovative Talents in Higher Education of Liaoning Province of China(LR2019008).

摘　　要：A specific peptide(EEEFDATR)that is released during the hydrolysis of Antarctic krill contains three glutamic acids that cover calcium-binding ligands,and might act as a potential role in enhancing the osteogenetic activity.In this study,EEEFDATR and calcium alone and in combination were examined for their effects on the proliferation,differentiation,and mineralization of MC3T3-E1 osteoblastic cells.Co-administration of CaCl_(2)and EEEFDATR showed a superior proliferation rate(70.94%)of MC3T3-E1 cells,which was about 2.07 and 1.74 times higher than CaCl_(2)and EEEFDATR alone,respectively.Osteoblast proliferation improvements were associated with increased cell cycle in S phase after co-administration of CaCl_(2)and EEEFDATR.The CaCl_(2)+EEEFDATR group further exhibited a significantly higher alkaline phosphatase(ALP)activity as compared to other groups(p<0.05)and showed obvious differentiation of MC3T3-E1 cells.The magnitude of mineralization in the extracellular matrix(calcium deposition)was also higher in MC3T3-E1 cells treated with combined administration,although EEEFDATR alone did not induce significant mineralization.These data suggest that co-administration of CaCl_(2)and EEEFDATR was superior to mono-administration in improving osteogenetic activity.

关 键 词：Antarctic krill peptide CALCIUM CO-ADMINISTRATION Osteogenetic activity 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]