一种新型的含筛孔八棱锥内构件泡沫分离设备的构建及其应用  

Construction and Application of a New Type of Foam Separation Equipment with the Hollow Octagonal Prismoid with Sieve Tray Inner Component

作　　者：薛静丽 方思含 张才亮 韩娟[1] 吴嘉聪 王蕾[2] 王赟[2] XUE Jingli;FANG Sihan;ZHANG Cailiang;HAN Juan;WU Jiacong;WANG Lei;WANG Yun(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;School of Chemistry&Chemical Engineering,Jiangsu University,Zhenjiang 212013,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏大学化学化工学院,江苏镇江212013

出　　处：《食品工业科技》2025年第1期104-113,共10页Science and Technology of Food Industry

基　　金：国家自然科学基金(22278191,22078133)。

摘　　要：目的:本研究设计了一种新型的含筛孔八棱锥内构件,用以强化泡沫排液,提高泡沫分离效率。方法:以重组β-葡萄糖苷酶(Glu-linker-ELP50-GB,GLEGB)发酵液为模型,考察内构件的结构参数(个数、间距、筛孔直径)和泡沫分离实验参数(温度、初始蛋白浓度、气体流速、装液量)对GLEGB富集比和回收率的影响。此外,基于弹性蛋白样多肽(elastin-like polypeptides,ELPs)的温敏性,对重组β-葡萄糖苷酶泡沫液进一步纯化,并通过圆二色光谱仪(CD)、紫外-可见分光光度计(UV-vis)和傅里叶-红外光谱仪(FT-IR)对纯化后GLEGB的结构进行分析。结果:在内构件数量为5、间距为78 mm、筛孔直径为2 mm、蛋白质初始浓度为0.3 mg/mL、温度为45℃、气体流速为200 mL/min、装液量为100 mL的最佳条件下,GLEGB的蛋白富集比为2.46±0.10是对照柱的1.33倍,对重组β-葡萄糖苷酶泡沫液进一步纯化后GLEGB的纯化倍数为37.25±0.60,此外,结果证明纯化过程不会影响GLEGB的结构。结论:本研究设计的含筛孔八棱锥内构件可以有效地提高从发酵液中分离GLEGB的富集比,为泡沫分离设备的设计、研究及工艺优化提供了新的研究思路。Objective:In this study,a novel type of the hollow octagonal prismoid with sieve tray inner component was designed to enhance liquid drainage from foam and improve the efficiency of foam separation.Methods:The model used in this work was a fermentation broth containing recombinant β-glucosidase(Glu-linker-ELP_(50)-GB,GLEGB).The effects of structural parameters of the internal component(number,spacing,and pore diameter)and operational parameters of the foam separation experiment(temperature,initial protein concentration,gas flow rate,and liquid loading volume)on the enrichment ratio and recovery rate of GLEGB were investigated.In addition,based on the temperature sensitivity of elastin�like polypeptides(ELPs),the recombinantβ-glucosidase foam solution was further purified.The structure of purified GLEGB was analyzed by circular dichroism spectrometer(CD),ultraviolet-visible spectrophotometry(UV-vis)and Fourier-transform infrared spectroscopy(FT-IR).Results:Under the optimal conditions of 5 internal components,78 mm spacing,2 mm pore diameter,0.3 mg/mL initial protein concentration,45℃ temperature,200 mL/min gas flow rate and 100 mL liquid loading volume,the protein enrichment ratio of GLEGB was 2.46±0.10,1.33 times that of the control column.After further purification of recombinant β-glucosidase foam solution,the purification ratio of GLEGB was 37.25±0.60.In addition,the results showed that the purification process did not affect the structure of GLEGB.Conclusion:The hollow octagonal prismoid with sieve tray inner component can effectively improve the enrichment ratio of GLEGB separation from fermentation liquid,which provides a new research idea for the design,research and process optimization of foam separation equipment.

关 键 词：泡沫分离 富集比 重组 Β-葡萄糖苷酶 内构件 

分 类 号：TS201.3[轻工技术与工程—食品科学]