酚酸基纳米金修饰电极检测食品中的亚硝酸盐和亚硫酸盐  

Determination of Nitrite and Sulfite in Foods by Phenolic Acid-based Gold Nanoparticles Electrode

作　　者：陈林林[1] 吴松遥 郝熙 李伟[1] 张铭 张海鹏 李昕彤 张娜[1] CHEN Linlin;WU Songyao;HAO Xi;LI Wei;ZHANG Ming;ZHANG Haipeng;LI Xintong;ZHANG Na(College of Food Engineering,Harbin University of Commerce,Harbin 150028,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《食品工业科技》2025年第1期274-282,共9页Science and Technology of Food Industry

基　　金：国家重点研发计划项目(2023YFD2100803);黑龙江省省属高等学校基本科研业务费科研项目(2023-KYYWF-1054)。

摘　　要：基于酚酸良好的还原性和无污染性建立酚酸基纳米金传感体系,以实现亚硝酸盐和亚硫酸盐的灵敏检测。采用不同酚酸还原氯金酸制备纳米金(Gold nanoparticles,AuNPs),通过紫外光谱和交流阻抗法获得还原效果最佳的酚酸为没食子酸(Gallic acid,GA)。利用电沉积法制备GA@AuNPs@Au电极,并由线性扫描伏安法(Linear scanning voltammetry,LSV)表征亚硝酸盐和亚硫酸盐的电化学行为。优化体系条件为:GA浓度1 mmol/L、孵育温度70℃、反应时间60 min、pH6.0磷酸盐缓冲溶液浓度0.15 mol/L。亚硝酸盐和亚硫酸盐浓度在0.001~7 mmol/L范围内与氧化峰电流分别呈良好的线性关系(R2>0.994),检出限分别为0.20μmol/L和0.24μmol/L(S/N=3)。该修饰电极的重现性和稳定性良好,可用于实际样品中亚硝酸盐和亚硫酸盐的同时测定。Based on the good reducibility and non-pollution of phenolic acid,the phenolic acid-based gold nanosensor system was established to realize sensitive detection of nitrite and sulfite.Different phenolic acids were used to reduce chlorauric acid to prepare gold nanoparticles(AuNPs).The optimal phenolic acid was gallic acid(GA)by ultraviolet spectrum and electrochemical impedance spectrum method.The GA@AuNPs@Au electrodes were prepared by electrodeposition and the electrochemical behaviors of nitrite and sulfite were characterized by linear scanning voltammetry(LSV).The optimized system conditions were as follows:GA concentration 1 mmol/L,incubation temperature 70℃,reaction time 60 min,pH6.0 phosphate buffer solution concentration 0.15 mol/L.The concentration of nitrite and sulfite in the range of 0.001 to 7 mmol/L showed a good linear relationship with the peak oxidation current(R 2>0.994),and the detection limits were 0.20μmol/L and 0.24μmol/L(S/N=3),respectively.The modified electrode has good reproducibility and stability,and it can be used for the simultaneous determination of nitrite and sulfite in real samples.

关 键 词：酚酸 纳米金 修饰电极 亚硝酸盐 亚硫酸盐 

分 类 号：TS207[轻工技术与工程—食品科学]