低共熔溶剂法提取葡萄皮籽多酚、黄酮及其抗氧化性研究  

A Study on the Extraction of Polyphenols and Flavonoids from Grape Skin Seeds and Its Antioxidant Properties by Deep Eutectic Solvent Process

作　　者：何彦芳 陈敬书 郭欢欢 褚文慧 王康威 侯旭杰[1] HE Yanfang;CHEN Jingshu;GUO Huanhuan;CHU Wenhui;WANG Kangwei;HOU Xujie(College of Food Science and Engineering,Tarim University/Production&Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang,Alar 843300;College of Information Engineering,Tarim University,Alar 843300)

机构地区：[1]塔里木大学食品科学与工程学院/南疆特色农产品深加工兵团重点实验室,阿拉尔843300 [2]塔里木大学信息工程学院,阿拉尔843300

出　　处：《食品工业》2024年第11期54-59,共6页The Food Industry

基　　金：新疆维吾尔自治区重点研发计划课题,慕萨莱思新产品研发及副产物综合利用技术研究(2022B02024-3)。

摘　　要：以和田红葡萄皮籽为原材料,采用低共熔溶剂法提取其多酚和黄酮类物质,并与80%乙醇为溶剂提取效果进行比较试验。以黄酮和多酚提取量为指标,经单因素试验和响应面优化试验,获得了最优的提取工艺条件,并分析了提取液对DPPH、ABTS+自由基的清除能力。结果表明,低共熔溶剂的组成为氯化胆碱-乳酸(1∶2),含水量为20%,最佳工艺条件为提取温度50℃、提取时间35 min、料液比1∶26(g/mL),此时黄酮平均提取量为57.46 mg/g,多酚平均提取量为79.81 mg/g。葡萄皮籽提取物表现出显著的抗氧化活性。This research utilized Hontan red grape peels and seeds as raw materials,and employed a deep eutectic solvent(DES)method to extract polyphenols and flavonoids,comparing the results with extraction using 80%ethanol as the solvent.Through single-factor experiments and response surface methodology optimization,the optimal extraction conditions were determined,and the scavenging ability of the extract on DPPH and ABTS+free radicals was analyzed.The results indicated that the optimal DES composition was choline chloride-lactic acid(1∶2)with a water content of 20%.The best extraction conditions were an extraction temperature of 50℃,an extraction time of 35 minutes,and a solid-to-liquid ratio of 1∶26(g/mL).Under these conditions,the average extraction yield of flavonoids was 57.46 mg/g,and the average extraction yield of polyphenols was 79.81 mg/g.The extract from grape peels and seeds exhibited significant antioxidant activity.

关 键 词：低共熔溶剂法 响应面优化试验 黄酮 多酚 

分 类 号：TS209[轻工技术与工程—食品科学]