婴幼儿配方食品中2’-岩藻糖乳糖和乳酰-N-新四糖的测定  

Determination of 2’-Fucosyllactose and Lacto-N-neotetraose in Infant Formula

作　　者：李雅玫 杨中 黄刚 刘俊 沈晓芳 李立[6] 吴凤琪 LI Yamei;YANG Zhong;HUANG Gang;LIU Jun;SHEN Xiaofang;LI Li;WU Fengqi(Food Inspection Center of Shenzhen Customs,Shenzhen 518000;Xinjiang Uygur Autonomous Region Institute of Analysis and Measurement,Uygur 830011;Comprehensive Technology Center of Huizhou Customs,Huizhou 516006;Technology Center of Chengdu Customs,Chengdu 516006;Jiangnan University,Wuxi 214122;Chinese Academy of Inspection and Quarantine,Beijing 100176)

机构地区：[1]深圳海关食品检验检疫技术中心,深圳518000 [2]新疆维吾尔自治区分析测试研究院,乌鲁木齐830011 [3]惠州海关综合技术中心,惠州516006 [4]成都海关技术中心,成都610041 [5]江南大学,无锡214122 [6]中国检验检疫科学研究院,北京100176

出　　处：《食品工业》2024年第11期325-329,共5页The Food Industry

摘　　要：建立高效液相色谱法测定婴幼儿配方食品中2’-岩藻糖乳糖(2’-fucosyllactose,2’-FL)和乳酰-N-新四糖(lacto-N-neotetraose,LNnt)的分析方法。样品经70℃的水提取,2-邻氨基苯甲酰胺(2-AB)衍生,得到稳定的OS-2AB衍生物,用乙腈稀释衍生后的提取液,采用高效液相色谱-荧光检测器检测,外标法定量。结果表明,在10.0~200μg/L浓度范围内,2’-FL和LNnt线性关系良好,相关系数均≥0.999。2’-FL和LNnt的检出限均为12.0 mg/100 g,定量限均为36.0 mg/100 g。考察空白奶粉样品添加3个浓度水平的回收率结果,回收率在90%~105%范围内,相对标准偏差为1.29%~3.77%。该方法灵敏度高、选择性好、准确可靠、操作简单,可用于婴幼儿配方食品中2’-FL和LNnt含量的同时检测。A method for simultaneous determination of 2’-fucosyllactose(2’-FL)and lacto-N-neotetraose(LNnt)in infant formula by HPLC(high performance liquid chromatography)was established.The samples were extracted with water at 70℃and derived from 2-o-aminobenzamide(2-AB)to obtain stable OS-2AB derivatives.The extracted solution was diluted with acetonitrile.The sample was detected by HPLC-fluorescence detector and quantified by external standard.In the concentration range of 10.0-200μg/L,there was a good linear relationship between 2’-FL and LNnt,and the linear correlation coefficients were greater than or equal to 0.999.The limits of detection of 2’-FL and LNnt were 12.0 mg/100 g,and the limits of quantitation were 36.0 mg/100 g.The recoveries of 2’-FL and LNnt in blank infant formula samples with 3 concentration levels were investigated.The recoveries of 2’-FL and LNnT were in the range of 90%-105%,and the relative standard deviations were 1.29%-3.77%.The method has high sensitivity,good selectivity,accuracy,reliability and simple operation,and can be used for the detection of 2’-FL and LNnt in infant formula food.

关 键 词：2’-岩藻糖乳糖 乳酰-N-新四糖 高效液相色谱法 婴幼儿配方食品 

分 类 号：TS216[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]