Low-magnitude high-frequency vibration reduces prostate cancer growth and extravasation in vitro  

作　　者：Amel Sassi Kimberly Seaman Xin Song Chun-Yu Lin Yu Sun Lidan You 

机构地区：[1]Institute of Biomedical Engineering,University of Toronto,M5S 3G9,Canada [2]Department of Mechanical&Industrial Engineering,University of Toronto,M5S 3G9,Canada [3]Department of Mechanical and Materials Engineering,Queen's University,K7L 3N6,Canada

出　　处：《Mechanobiology in Medicine》2024年第4期83-96,共14页力学生物学与医学（英文）

基　　金：supported by the Natural Science and Engineering Research Council(NSERC,Grant No:06465-14).

摘　　要：Prostate cancer(PCa)continues to rank among the most common malignancies in Europe and North America with significant mortality rates despite advancements in detection and treatment.Physical activity is often recommended to PCa patients due to its benefits in preventing disease recurrence and managing treatment-related side effects.However,physical activity may be challenging for elderly or bedridden patients.As such,vibration therapy has been proposed as a safe,effective,and easy to perform alternative treatment that may confer similar effects as physical exercise.Specifically,low-magnitude high frequency(LMHF)vibration has been shown to decrease breast cancer extravasation into the bone and reduce other types of cancer proliferation by impacting cell viability.Here,we investigated the effects of daily application of LMHF vibration(0.3 g,60 Hz,1 hour/day for 3 days)on prostate cancer growth and bone metastasis in vitro.Our findings suggest that LMHF vibration significantly reduces colony formation through a decrease in cell growth and proliferation.Moreover,using a 3D cell culture model,LMHF vibration significantly reduces PC3 spheroid size.Additionally,LMHF vibration reduces PCa cell extravasation into the bone microenvironment through the stimulation of osteocytes and subsequent osteocyte-endothelial cell cross talk.These findings highlight the potential of LMHF vibration for managing PCa growth and metastasis.

关 键 词：Low-magnitude high-frequency vibration Mechanical loading Vibration Prostate cancer Bone metastasis OSTEOCYTE Microfluidic SPHEROID 

分 类 号：R737.25[医药卫生—肿瘤]