乌梅多糖提取工艺、理化性质及其体外功能活性  

Extraction Process,Physicochemical Properties,and in vitro Functional Activity of Polysaccharide from Fructus mume

作　　者：曹田欣 闫小娟 张海悦[1] CAO Tianxin;YAN Xiaojuan;ZHANG Haiyue(School of Chemistry and Life Sciences,Changchun University of Technology,Changchun 130012,Jilin,China;Jilin Fusheng Special Medical Food Co.,Ltd.,Changchun 130012,Jilin,China)

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012 [2]吉林省富生特医食品有限公司,吉林长春130012

出　　处：《食品研究与开发》2024年第24期101-110,共10页Food Research and Development

基　　金：吉林省科技厅重点医药项目(20200708058YY)。

摘　　要：该文主要研究乌梅多糖的提取工艺及其体外功能活性,考察超声功率、提取时间、料液比、提取温度对乌梅多糖得率的影响,采用响应面优化出乌梅多糖的最优提取工艺为料液比1∶22(g/mL)、提取温度39℃、提取时间67min、超声功率160W,乌梅多糖的平均得率高达9.7%,与预测值相近,采用sevage法脱蛋白,对乌梅多糖的化学组成分析,扫描电镜结果显示其为片层结构,红外光谱结果表明其为α‐构型呲喃糖,紫外光谱显示乌梅多糖不含有核酸、蛋白质、色素等。刚果红试验表明乌梅多糖不含三股螺旋结构,抗氧化活性试验结果表明乌梅多糖具有良好的抗氧化能力,乌梅多糖浓度为5mg/mL时,对甘氨胆酸钠的结合率达到45.73%,对牛磺胆酸钠的结合率为32.50%;乌梅多糖浓度为10mg/mL时,胰脂肪酶抑制率为49.7%,说明乌梅多糖具有良好的降血脂能力。The extraction process and in vitro functions of the polysaccharide from Fructus mume were studied.The extraction conditions including ultrasonic power,extraction time,solid‐to‐liquid ratio,and extraction tempera‐ture were optimized by response surface methodology.The optimal extraction process was a solid‐liquid ratio of 1∶22(g/mL),extraction temperature of 39℃,extraction time of 67 min,and ultrasonic power of 160 W.The aver‐age yield of polysaccharide extracted from Fructus mume under the optimum conditions was 9.7%,which was close to the predicted value.The results of scanning electron microscopy showed that the extracted polysaccha‐ride appeared a lamellar structure,and the infrared spectrum showed that the polysaccharide wasα‐morpho‐pyranose.UV results showed that there was no nucleic acid,protein or pigment in the polysaccharide.The results of Congo red test showed that the polysaccharide had no triple helix structure and exerted high antioxidant activ‐ity.The polysaccharide at 5 mg/mL showcased the binding rates of 45.73%and 32.50%to sodium glycocholate and sodium taurocholate,respectively.The polysaccharide at 10 mg/mL demonstrated the inhibition rate of 49.7%on pancreatic lipase.The results suggested that Fructus mume polysaccharide had a lipid‐lowering activity.

关 键 词：乌梅 多糖 水提醇沉 理化性质 抗氧化活性 降血脂活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]