雪莲菌中产S层蛋白乳酸杆菌的筛选及黏附性能  

Screening and Adhesion Properties of S-layer Protein Producing Lactobacillus from Tibet Kefir

作　　者：程天天 李佳乐 付梦琪 李美琦 桑亚新[1] 高洁 Cheng Tiantian;Li Jiale;Fu Mengqi;Li Meiqi;Sang Yaxin;Gao Jie(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,Hebei)

机构地区：[1]河北农业大学食品科技学院,河北保定071000

出　　处：《中国食品学报》2024年第11期32-39,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：河北省自然科学基金项目(C2021204061)。

摘　　要：目的:大量研究表明乳酸杆菌S层蛋白(SLP)具有黏附功能,包括助力菌体对肠上皮细胞的黏附或与细胞表面成分的稳定结合。本文旨在探究雪莲菌中乳酸杆菌SLP的黏附性能,评估其对雪莲菌中微生物间聚集及生物膜形成的影响。方法:采用传统分离手段及酸性氯化锂提取法从雪莲菌中筛选产SLP菌株,通过微生物溶剂黏附(MATS)法对比分析SLP对菌体表面疏水性及表面电荷的影响,最后通过测定乳酸杆菌细胞的自聚集率及其与酵母菌的共聚集率和生物膜形成能力分析SLP对菌体间聚集的作用。结果:从雪莲菌中分离筛选到19株产SLP乳酸杆菌,所产SLP分子质量均为70 ku左右,其中最高产量达1.16 g/mL。通过对比去除SLP前、后的菌体表面特性和聚集能力,发现去除SLP造成菌体平均自聚集率与共聚集率分别降低1.63%~7.56%和0.96%~10.63%,混合生物膜生长所对平均吸光度值减少范围为0.52~1.13 nm。结论:雪莲菌中富含高产SLP菌株,且多数为其优势菌相。作为影响聚集和黏附能力的重要物质,SLP在雪莲菌微生物群生物膜形成和稳定繁殖方面发挥着重要作用。Objective:The surface layer protein(SLP)of Lactobacillus,possesses adhesive functionalities,including facilitating the adherence of bacterial cells to intestinal epithelial cells or stable binding to surface constituents of cells.This study aimed to explore the adhesion properties of the SLP of Lactobacillus in Tibet kefir and evaluate its impact on the aggregation among microorganisms and biofilm formation.Methods:SLP-producing strains were selected from Tibet kefir using conventional separation techniques and an acidic lithium chloride extraction method.The impact of SLP on the hydrophobicity and surface charge of bacterial cells was investigated by employing the microbial adhesion to solvents(MATS)assay.Finally,the self-aggregation rate of Lactobacillus,their co-aggregation rate with yeasts,and the capacity for biofilm formation were determined to assess the influence of SLP on microbial aggregation and biofilm formation within the Tibet kefir microbiota.Results:19 strains of SLP-producing Lactobacillus from Tibet kefir,with SLPs exhibiting an average molecular weight of approximately 70 ku,and the highest production level reaching 1.16 g/mL.By comparing the surface characteristics and aggregation abilities of bacteria before and after the removal of SLP,it was found that the removal of SLP caused the average auto-aggregation rate and co-aggregation rate of bacteria to decrease by 1.63%-7.56%and 0.96%-10.63%,respectively,and the reduction range of the average absorbance value corresponding to the growth of the mixed biofilm was 0.52-1.13 nm.Conclusion:Tibet kefir was found to harbor an abundant population of high SLP-producing strains,with the majority representing dominant microbial species.As a critical component governing aggregation and adhesion abilities,SLP plays a pivotal role in the biofilm formation and stable propagation of the microbial community within Tibet kefir.

关 键 词：雪莲菌 S层蛋白 聚集 黏附 生物膜 

分 类 号：TS201.3[轻工技术与工程—食品科学]