Characteristics of cold plasma treatment and enzymatic hydrolysis on IgG/IgE-binding ability of β-lactoglobulin  

作　　者：Zhi-Wei Liu Lin-Lin Zhang Ying-Xue Zhou Pan-Pan Tang Yi-Cheng Tan Jun-Hu Cheng Amin Mousavi Khaneghah Rana Muhammad Aadil 

机构地区：[1]College of Food Science and Technology,Hunan Agricultural University,Changsha,410128,China [2]School of Food Science and Engineering,South China University of Technology,Guangzhou,510641,China [3]Department of Fruit and Vegetable Product Technology,Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute,36 Rakowiecka St.,02-532,Warsaw,Poland [4]National Institute of Food Science and Technology,University of Agriculture,Faisalabad,38000,Pakistan

出　　处：《Food Bioscience》2022年第6期1696-1705,共10页食品生物科学（英文）

基　　金：The foundation of the Leading Plan of High-tech and Innovation Industry Technology of Hunan Province(2022GK4014).

摘　　要：Characteristics of cold plasma (CP) treatment and enzymatic (papain and alcalase) hydrolysis on IgG/IgE-binding ability β-lactoglobulin (β-LG) were compared, which is analyzed by immunoblot, WB, ELISA, and LC-MS/MS analysis. Results indicated that CP treatment exhibit similar characteristic to enzymatic hydrolysis to cleave the polypeptides chain of β-LG for reduction of its IgG/IgE binding ability. A similar tendency of decline in IgG/IgE binding capacity of β-LG after CP treatment was achieved in comparison with alcalase hydrolysis, which was reduced by 57.69% (IgG) and 56% (IgE) (for 4 min CP treatment) and 61.89% (IgG) and 27.54% (IgE) for alcalase hydrolysis (75 min), while no significant difference was detected for papain hydrolysis. Immunoblot and LC-MS/MS analysis confirmed that the antigenicity reduction was ascribed to the breakdown of the IgG/IgE linear epitopes of β-LG. Further combined with CP treatment (1 min) with alcalase hydrolysis (75 min), the reduction of antigenicity of β-LG reached 68.46% (IgG) and 59.26% (IgE). This study suggests that CP treatment is a promising alternative to enzymatic hydrolysis for hypoallergenic protein-producing.

关 键 词：Β-LACTOGLOBULIN Cold plasma Enzyme hydrolysis ANTIGENICITY 

分 类 号：TS20[轻工技术与工程—食品科学]