蜂胶油提物的制备及抗氧化活性研究  

Preparation and antioxidant activity of propolis oil extracts

作　　者：彭正菊 谌迪 张岑 卢文静 喻宏应 郭慧媛[3] 蒋晗 肖朝耿[2] PENG Zhengju;CHEN Di;ZHANG Cen;LU Wenjing;YU Hongying;GUO Huiyuan;JIANG Han;XIAO Chaogeng(College of Life Sciences,China Jiliang University,Hangzhou 310018,China;Institute of Food Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国计量大学生命科学学院,浙江杭州310018 [2]浙江省农业科学院食品科学研究所,浙江杭州310021 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《浙江农业学报》2024年第10期2338-2346,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(32201930);浙江省农业科学院国际合作/港澳台专项经费资助项目(蜂胶油凝胶的制备及功效机理研究)。

摘　　要：蜂胶是一种良好的药食两用天然产品,具有广泛的生物活性。为探索山茶油提取对蜂胶中活性成分及抗氧化活性的影响,该研究以总黄酮为评价指标,通过单因素及响应面实验获得蜂胶提取最佳工艺条件,并对油提物的DPPH清除能力和铁离子还原能力等抗氧化活性进行了评价,最后以蜂胶中典型的脂溶性高良姜素为例,利用分子对接对其抗氧化活性机制进行了探究。结果表明,提取的最佳工艺为:温度41℃,液固比16∶1 mL·g^(-1),提取时间31 h,此时总黄酮的含量为35.59 mg·g^(-1)。在该工艺下获得的油提物对DPPH清除率的IC_(50)值为12.48μg·mL^(-1),总抗氧化能力(FRAP)为3.70 mmol·g^(-1)。此外,分子对接结果表明,蜂胶中典型脂溶性成分高良姜素与蜂胶的抗氧化作用密切相关。Propolis is a good medicinal and edible natural product,which have a wide range of biological activities.The purpose of this study was to explore the effect of camellia oil extraction on the active components and antioxidant activity in propolis.Firstly,total flavonoids were used as the evaluation index,this study obtained the optimal process conditions for propolis extraction by one-way and response surface experiments.Then,we evaluated the antioxidant activity of the oil extracts including DPPH scavenging ability and ferric iron reduction ability.Finally,the mechanism of the antioxidant activity of galangin,a typical fat-soluble compound in propolis,was investigated using molecular docking was explored.The results showed that the optimal process was as follows:extraction temperature 41℃,liquid-solid ratio 16∶1 mL·g^(-1),extraction time 31 h,at which the content of total flavonoids was 35.59 mg·g^(-1).The IC50 value of the oil extracts obtained under this process was 12.48μg·mL-1 for DPPH scavenging,and the total antioxidant capacity(FRAP)was 3.70 mmol·g^(-1).In addition,the molecular docking results indicated that galangin,a typical fat-soluble component of propolis,was closely related to the antioxidant effect of propolis.

关 键 词：蜂胶 总黄酮 响应面 抗氧化 分子对接 

分 类 号：TS201[轻工技术与工程—食品科学]