Phytosterol of lotus seed core powder alleviates hypercholesterolemia by regulating gut microbiota in high-cholesterol diet-induced C57BL/6J mice  

作　　者：Dongmin Liu Jiating Pi Bo Zhang Hongliang Zeng Changzhu Li Zhihong Xiao Fang Fang Miao Liu Na Deng Jianhui Wang 

机构地区：[1]School of Food Science and Bioengineering,Changsha University of Science&Technology,Changsha,410114,China [2]Research Institute of Chinese Medicine,Hunan Academy of Chinese Medicine,Changsha,Hunan,410013,China [3]State Key Laboratory of Utilization of Woody Oil Resources,Hunan Academy of Forestry,Changsha,410018,China [4]Hunan Provincial Engineering Technology Research Center of Intelligent Manufacturing and Quality Safety of Xiang Flavoured Compound Seasoning for Chain Catering,Liuyang,410023,China

出　　处：《Food Bioscience》2023年第1期644-655,共12页食品生物科学（英文）

基　　金：funded by Hunan Provincial Science Fund for Distinguished Young Scholars(2021JJ10007);the Research and Development Program in Key Areas of Hunan Province(2021NK2015,2020NK2027,2019SK2121);Hunan Scientific Talents Promotion Program supported by Hunan Association for Science and Technology(2019 TJ-Q01);the Research and Development Program in Key Areas of Hunan Province(2020NK2027).

摘　　要：The present study aimed to investigate the regulatory mechanisms of lotus seed core powder phytosterol extract(LSP-PE)on hypercholesterolemia and intestinal microbiota in high-cholesterol diet(HCD)-induced C57BL/6 J mice.Gavage with LSP-PE reduced levels of serum total cholesterol(TC),ratio of low-density lipoprotein cholesterol(LDL-C)to high-density lipoprotein cholesterol(HDL-C),hepatic TC,hepatic triglyceride(TG),hepatic LDL-C,hepatic very low-density lipoprotein(VLDL),fecal TC and fecal total bile acid(TBA),while augmented hepatic HDL-C content.In contrast with HCD group,high-dose LSP triggered significant diminutions of serum ALT,serum AST,hepatic ALT and hepatic AST levels in HCD-fed mice,which were 16.49,28.94,43.42,and 44.47%,respectively,thus remarkably ameliorating the pathological changes of liver and small intestine.Indepth genetic research found that LSP was effective in down-regulating the expression of cholesterol metabolism related genes including Niemannpick C1-like protein 1(NPC1L1),acyl-coA cholesterol acyl-transferase 2(ACAT2),microsomal triglyceride transporter(MTP)and ATP binding cassette subfamily G member 5(ABCG5).Moreover,LSP-PE could restore the disorder of intestinal flora via augmenting the Actinobacteria abundance as well as decreasing the Firmicutes/Bacteroidetes ratio and abundances of Proteobacteria and Verrucomicrobia to regulate cholesterol metabolism-related genes.In summary,LSP-PE possessed excellent cholesterol-lowering activity by positively regulating the gut microbiota and the expression levels of genes related to cholesterol absorption and metabolism.Therefore,this work suggested LSP phytosterol as a potential supplement to alleviate the symptoms of hypercholesterolemia.

关 键 词：Lotus seed core powder PHYTOSTEROL HYPERCHOLESTEROLEMIA Gut microbiota Cholesterol metabolism 

分 类 号：TS201[轻工技术与工程—食品科学]