Electrochemiluminescence Imaging of Liposome Permeabilization by an Antimicrobial Peptide:Melittin  

作　　者：Fatma Ben Trad Jérôme Delacotte Manon Guille-Collignon Frédéric Lemaître Stéphane Arbault Neso Sojic Fabienne Burlina Eric Labbé Olivier Buriez 

机构地区：[1]PASTEUR,Département de Chimie,Ecole Normale Supérieure,PSL University,Sorbonne Université,CNRS,75005 Paris,France [2]Univ.Bordeaux,CNRS,Bordeaux INP,CBMN,UMR 5248,F-33600 Pessac,France [3]Univ.Bordeaux,CNRS,Bordeaux INP,ISM,UMR 5255 CNRS,33400 Talence,France [4]Sorbonne Université,Ecole Normale Supérieure,PSL University,CNRS,Laboratoire des Biomolécules(LBM),75005 Paris,France

出　　处：《Chemical & Biomedical Imaging》2023年第1期58-65,共8页化学与生物医学影像（英文）

基　　金：supported in parts by CNRS UMR 8640,Ecole Normale Supérieure,PSL University and Sorbonne Université.F.B.T.thanks the doctoral school ED388“Chimie Physique et de Chimie Analytique de Paris Centre”for a PhD grant.We thank Gilles Clodic(MS3U platform,Sorbonne Université)for MALDI-TOF mass spectrometry analysis.N.S.acknowledges the financial support from Agence Nationale de la Recherche(ELISE-ANR-21-CE42).

摘　　要：The permeabilization of liposomes by melittin,an antimicrobial peptide(AMP),has been studied by an electrochemiluminescence(ECL)imaging strategy.The methodology consisted ffrst of encapsulating ECL reagents in sealed giant asymmetrical liposomes(100μm in diameter)made of DOPG/DOPC phospholipids(i.e.,1,2-dioleoyl-sn-glycerol-3-phospho-(1′-rac-glycerol)sodium salt/1,2-dioleolyl-sn-glycero-3-phosphocholine).Then liposomes were placed on an indium tin oxide electrode coated with poly-L-lysine to avoid any membrane poration/permeabilization through polarization of the electrode surface.Finally,the addition of melittin(from 10μM to 100 nM in concentration)enabled the permeabilization of the lipid membrane followed by the liposome content release and subsequent light generation through the ECL reagents oxidation processes.Interestingly,at a melittin concentration of 10μM,two successive leakages occurring on the same liposome could be imaged.Combination of ECL and photoluminescence imaging allowed comprehensive monitoring of the permeabilization and content release of a single liposome.This ECL imaging approach opens interesting perspectives to characterize the instant release of vesicle content upon permeabilization by AMPs or other membrane-active species.

关 键 词：ELECTROCHEMILUMINESCENCE LIPOSOME ECL imaging PERMEABILIZATION Membrane active peptide MELITTIN 

分 类 号：O646[理学—物理化学]