QuEChERS-固相萃取-高效液相色谱-串联质谱法测定预制食品中丙烯酰胺的含量  

Determination of acrylamide in prepared foods by QuEChERS-solid phase extraction-high performance liquid chromatography-tandem mass spectrometry

作　　者：贝荣廷 何焜鹏 刘嘉欣 吴平谷[3] 陈宝欣 郑泽洋 陈颖怡 林丹[1] 冯志强[1] 庄俊钰[1] BEI Rong-Ting;HE Kun-Peng;LIU Jia-Xin;WU Ping-Gu;CHEN Bao-Xin;ZHENG Ze-Yang;CHEN Ying-Yi;LIN Dan;FENG Zhi-Qiang;ZHUANG Jun-Yu(Guangdong Provincial Public Laboratory of Food Industry,Guangdong Food Industry Institute Co.,Ltd.,Guangdong Food Quality Supervision and Inspection Station,Guangzhou 511442,China;Guangdong Provincial Institue of Pubilc Health,Guangzhou 511430,China;Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China)

机构地区：[1]广东省食品工业公共实验室,广东省食品工业研究所有限公司,广东食品质量监督检验站,广州511442 [2]广东省公共卫生研究院,广州511430 [3]浙江省疾病预防控制中心,杭州310051

出　　处：《食品安全质量检测学报》2024年第24期10-17,共8页Journal of Food Safety and Quality

基　　金：广东省重点研发计划项目(2019B020212001)。

摘　　要：目的建立一种测定预制食品中丙烯酰胺含量的QuEChERS-固相萃取-高效液相色谱-串联质谱法(solid phase extraction-high performance liquid chromatography-tandem mass spectrometry,SPE-HPLC-MS/MS)。方法采用水提法,结合QuEChERS以及CARB/SCX/PSA固相萃取小柱对预制食品中丙烯酰胺进行提取净化,同时借助HPLC-MS/MS进行测定,内标法定量。结果在5~500 mg/L范围内,线性相关系数大于0.997,方法的定量限为10.0μg/kg;在3个添加水平(20、40、100μg/kg)下的回收率在86.58%~108.84%,相对标准偏差在1.60%~5.42%。结论该方法简化了净化步骤,缩短前处理时间,节约成本,提高效率,且方法灵敏度高、准确度和精密度好,可应用于预制食品中丙烯酰胺含量的批量测定。Objective To establish a QuEChERS-solid phase extraction-high performance liquid chromatography-tandem mass spectrometry(SPE-HPLC-MS/MS)method for the determination of acrylamide in prepared food.Methods The acrylamide in prepared foods was extracted by water,and the extract was purified by combination with QuEChERS and CARB/SCX/PSA SPE columns.The analyte was quantified by HPLC-MS/MS with an internal standard.Results In the range of 5–500 mg/L,the correlation coefficient of linear regression was greater than 0.997,and the limit of quantitative the method was 10.0µg/kg.The recovery rates were 86.58%–108.84%at 3 added levels(20,40,100μg/kg)with standard deviations between 1.60%–5.42%.Conclusion This method can simplify the purification step,shorten the pretreatment time,save the cost and improve the efficiency with high sensitivity,good accuracy and precision,and can be applied to the batch determination of acrylamide content in prepared food.

关 键 词：丙烯酰胺 QUECHERS 固相萃取 预制食品 高效液相色谱-串联质谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]