响应面法优化黑豆硒蛋白的提取工艺  

Optimization of Extraction Process of Black Bean Selenoprotein by Response Surface Methodology

作　　者：祁蒙 王建安[2] 王倩玉 杜小平 贺博 张军 QI Meng;WANG Jian’an;WANG Qianyu;DU Xiaoping;HE Bo;ZHANG Jun(Ankang R&D Center for Se-enriched Products,Ankang 725000,China;Ankang Vocational and Technical College,Ankang 725000,China;Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]安康市富硒产品研发中心,陕西安康725000 [2]安康职业技术学院,陕西安康725000 [3]西北民族大学生物医学研究中心,甘肃兰州730030

出　　处：《现代食品》2024年第21期60-64,共5页Modern Food

基　　金：农业农村部杂粮加工重点实验室/四川省杂粮产业化工程技术研究中心开放课题资助项目“天然硒与外源硒对大豆营养成分的影响初探”(2023CC006);陕西省重点研发计划项目“基于高效液相色谱-电感耦合等离子体质谱法测定富硒农产品中硒形态的研究”(2024NC-YBXM-170);安康市科学技术研究发展计划项目“典型富硒植物中硒蛋白的提取及抗氧化活性研究”(AK2023-GY-09)。

摘　　要：黑豆具有较强的硒富集能力和较高的蛋白含量,因此黑豆硒蛋白成为人体补充硒元素的优质食物来源。为提高黑豆硒蛋白的提取率,本文以富硒黑豆为研究对象,采用超声波辅助碱提法提取黑豆硒蛋白,以黑豆蛋白提取率为指标设计单因素试验,考察NaOH浓度、提取时间、料液比和超声功率对黑豆硒蛋白提取率的影响,结合响应面法优化黑豆硒蛋白提取工艺。结果表明,3个因素对黑豆硒蛋白提取率的影响顺序为料液比>NaOH浓度>提取时间。黑豆硒蛋白最佳提取工艺条件为NaOH浓度0.16 mol·L^(-1),提取时间4.00 h,料液比1∶40,超声波功率250.00 W,在此条件下黑豆硒蛋白提取率达到59.19%。本研究为黑豆硒蛋白资源的开发提供了参考。Black beans have strong selenium enrichment ability and high protein content,making black bean selenoprotein a high-quality food source for supplementing selenium elements in the human body.In order to improve the extraction rate of selenoprotein from black beans,this study focuses on selenium rich black beans and uses ultrasonic assisted alkaline extraction to extract selenoprotein from black beans.A single factor experiment is designed with the extraction rate of black beans as the indicator to investigate the effects of NaOH concentration,extraction time,solid-liquid ratio,and ultrasonic power on the extraction rate of selenoprotein from black beans.The response surface methodology is used to optimize the extraction process of selenoprotein from black beans.The results showed that the order of the influence of the three factors on the extraction rate of black bean selenoprotein was material to liquid ratio>NaOH concentration>extraction time.The optimal extraction conditions for black bean selenium protein are NaOH concentration of 0.16 mol·L^(-1),extraction time of 4.00 h,solid-liquid ratio of 1∶40,ultrasonic power of 250.00 W.Under these conditions,the extraction rate of black bean selenium protein reaches 59.19%.This study provides a reference for the development of selenium protein resources in black beans.

关 键 词：黑豆 硒蛋白 提取工艺 响应面法 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程]