Gliadin nanoparticles stabilized by sodium carboxymethyl cellulose as carriers for improved dispersibility,stability and bacteriostatic activity of Natamycin  

作　　者：Zhaojun Ban Fangyuan Chen Lingling Liu Shuang Zhang Lijun Wang Hongpeng Wang Luyin Wang Yi Zhu 

机构地区：[1]Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology of Farm Products,Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing,School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou,310023,China [2]School of Environmental and Natural Resources,Zhejiang University of Science and Technology,Hangzhou,310023,China [3]Aksu Youneng Agricultural Technology Co.,Ltd,Aksu,843001,China

出　　处：《Food Bioscience》2023年第3期887-895,共9页食品生物科学（英文）

基　　金：supported by the National Science Foundation of China[grant number 32172268];Zhejiang Provincial Key Research and Development Program[grant numbers 2022C04039 and 2021C02015];Zhejiang Public Welfare Technology Research Project[grant number LQY19E030001];the National Major Increase or Decrease Project-Construction of the Sustainable Utilization Capacity of Famous Traditional Chinese Medicine Resources[grant number 2060302];the Opening-fund of Key Laboratory of Storage of Agricultural Products of Ministry of Agriculture and Rural Affairs[grant number Kf202208];Research Project on Postgraduate Teaching Reform of Zhejiang University of Science and Technology[grant number 2020yjsjg03];Fundamental Research Funds for the Zhejiang University of Science and Technology[grant number 2021JLZD006]。

摘　　要：There is increasing interest in the development of Natamycin(Nata)containing ingredients appropriate as substitutes for chemical food preservatives,due to its safe and efficient antimycotic effects.This work reported that gliadin(Gli)could effectively perform as nano-vehicles to enhance Nata’s water dispersibility and stability.Nata-Gli nanoparticles(NPs)were prepared by adjusting the solution pH and anti-solvent method using the amphiphilicity of Gli.The encapsulation rate of NPs could reach 85.5%,and the loading rate was 8.6%.Nata-Gli/CMC nanocomposite system was prepared using sodium carboxymethylcellulose(CMC)as an antisolvent.Compared with the Nata-Gli NPs,the spherical structure of the particles with CMC addition is more apparent and more homogeneous.The bacteriostatic properties of the Nata-Gli/CMC nanocomposite system against Penicillium expansum were tested in vitro and in vivo.The results in vitro results confirmed that the application of Nata-Gli/CMC nanocomposite system improved the water dispersibility of natamycin and thus enhanced the bacteriostatic activity of natamycin.The results in vivo indicated that Nata-Gli/CMC nanocomposite system reduced the occurrence of rot and mildew in inoculated kumquat.The findings provide an effective strategy to provide a new idea for applying Nata and provide a reference for developing the post-harvest colloidal delivery system.

关 键 词：NATAMYCIN Gliadin nanoparticles ANTIFUNGAL KUMQUAT 

分 类 号：TS201.2[轻工技术与工程—食品科学]