毛细管柱气相色谱内标法检测蒸馏白酒中甲醇和乙酸乙酯的含量  

Determination of Methyl Alcohol and Ethyl Acetate in Distilled Baijiu by Capillary Column Gas Chromatography with Internal Standard

作　　者：李洋 熊文 张杰 LI Yang;XIONG Wen;ZHANG Jie(Enshi Center for Disease Control and Prevention,Enshi 445000,China)

机构地区：[1]恩施市疾病预防控制中心,湖北恩施445000

出　　处：《食品安全导刊》2024年第36期31-36,共6页China Food Safety Magazine

摘　　要：目的:建立毛细管柱气相色谱内标法测定蒸馏白酒中甲醇和乙酸乙酯含量的方法。方法:以叔戊醇为内标物质,利用气相色谱-氢火焰离子化检测器和INNOWAX色谱柱对市售蒸馏白酒中的甲醇和乙酸乙酯进行检测,内标法定量。结果:通过优化色谱条件,甲醇和乙酸乙酯可以实现有效分离;两种物质与叔戊醇的浓度比在各自线性范围内线性关系良好,相关系数分别为0.999 9和0.999 5;在低、中、高3个加标水平下,两种样品的平均回收率在96.9%~99.4%,相对标准偏差在0.66%~2.03%。结论:该方法灵敏度好,准确性及分析效率高,适用于检测蒸馏白酒中甲醇和乙酸乙酯的含量。Objective:To establish a capillary column gas chromatography internal standard method for the determination of methyl alcohol and ethyl acetate in distilled Baijiu.Method:Using tert-amyl alcohol as internal standard material,methanol and ethyl acetate in distilled Baijiu were determined by gas chromatography-hydrogen flame ionization detector and INNOWAX column.Result:By optimizing chromatographic conditions,methanol and ethyl acetate could be effectively separated;the concentration ratio between the two substances and tert-amyl alcohol had a good linear relationship in their respective linear ranges,and the correlation coefficients were 0.999 9 and 0.999 5,respectively;the average recoveries of the two samples were 96.9% to 99.4% and the relative standard deviations were 0.66% to 2.03% under the three levels of low,middle and high.Conclusion:The method has good sensitivity,high accuracy and high analytical efficiency,and is suitable for the determination of methyl alcohol and ethyl acetate in distilled Baijiu.

关 键 词：气相色谱 蒸馏白酒 甲醇 乙酸乙酯 

分 类 号：TS262.3[轻工技术与工程—发酵工程] O657.71[轻工技术与工程—食品科学与工程]