Nanobody-based food allergen surveillance:current status and prospects  

作　　者：Yi Wang Sihao Wu Ang Li Huan Lv Xuemeng Ji Yaozhong Hu Shuo Wang 

机构地区：[1]Tianjin Key Laboratory of Food Science and Health,School of Medicine,Nankai University,Tianjin,China

出　　处：《Food Quality and Safety》2024年第2期227-238,共12页食品品质与安全研究（英文版）

基　　金：funded by the National Natural Science Foundation of China(No.32272403);the National Key Research and Development Program of China(No.2022YFF1102400).

摘　　要：The incidence of food allergy has increased in recent decades,posing drastic risks to sensitive individuals,leading to mild to severe allergic symptoms.There is still no effective immune therapeutic strategy for food allergy that addresses accurate analytical methods to indicate the presence of allergens to prevent exposure of sensitive individuals.Currently,the most commonly applied detection method is immunoassay developed with food allergen-specifc antibodies,especially the conventional formats of monoclonal antibodies(mAbs)and polyclonal antibodies(pAbs),which serve as dominant detection reagents for food allergen analysis,although with the disadvantages of being labor-intensive,costly,batch differences,and signifcant cross-reaction,etc.Camelid-derived nanobodies(Nbs)have attracted tremendous attention to explore their application in food hazard analysis because of robust characteristics like unique paratopes,high stability and affnity that strongly contribute to the benefcial effect.However,extensive analysis is needed to validate the potential use of Nbs as detection reagents and the advantages for food allergen surveillance.Previous reports have demonstrated the potential of Nbs for immunoassay development against food allergens,such as macadamia allergen Mac i 1,peanut allergens Ara h 1 and Ara h 3,lupin allergen Lup an 1,milk allergenβ-lactoglobulin,etc.In this review,we comprehensively summarize the structural and biochemical properties of Nbs that beneft the application of Nb-based immunoassay,as well as the representative detection strategies,to provide research data for newly developed Nb formats for food allergen analysis,and investigate the future establishment of Nb-based surveillance against major food allergens.

关 键 词：Food allergy food allergen NANOBODY IMMUNOASSAY 

分 类 号：Q78[生物学—分子生物学]