枯草芽孢杆菌JZXJ-7对副溶血性弧菌感染小鼠肠道功能及菌群的影响  

Effect of Bacillus subtilis JZXJ-7,a Strain Isolated from Shrimp Paste,on Colonic Function and Microbial Community in Mice Infected with Vibrio parahaemolyticus

作　　者：周奎 王润东 宫梦学 牟景远 邓义佳 李学鹏 张宇昊[3] 励建荣 ZHOU Kui;WANG Rundong;GONG Mengxue;MOU Jingyuan;DENG Yijia;LI Xuepeng;ZHANG Yuhao;LI Jianrong(College of Food Science and Engineering,Lingnan Normal University,Zhanjiang 524048,China;College of Food Science and Engineering,Bohai University,Jinzhou 121013,China;College of Food Science,Southwest University,Chongqing 400715,China)

机构地区：[1]岭南师范学院食品科学与工程学院,广东湛江524048 [2]渤海大学食品科学与工程学院,辽宁锦州121013 [3]西南大学食品科学学院,重庆400715

出　　处：《食品科学》2025年第3期90-101,共12页Food Science

基　　金：国家自然科学基金青年科学基金项目(32202134;32201929);2025年广东省自然科学基金面上项目(2025A1515011748);岭南师范学院高层次人才专项(ZL2415);广东省科技创新战略专项(2022A05035)。

摘　　要：为探究枯草芽孢杆菌(Bacillus subtilis)JZXJ-7对副溶血性弧菌(Vibrio parahaemolyticus)感染小鼠的保护作用,本实验将28只雄性C57BL/6J小鼠随机分为4组,使用标准毒力株ATCC33847构建副溶血性弧菌感染模型,干预组分别灌胃低(10^(6) CFU)和高剂量(10^(8) CFU)JZXJ-7,对照组和模型组给予等体积生理盐水。检测各组小鼠疾病活动指数(disease activity index,DAI)、肠道(回肠、盲肠和结肠)副溶血性弧菌载量、结肠病理损伤情况、结肠细胞因子含量、氧化应激抑制能力和微生物群落组成变化。结果显示,高剂量JZXJ-7对副溶血性弧菌感染小鼠的保护作用优于低剂量,高剂量JZXJ-7可以显著降低副溶血性弧菌感染小鼠的DAI、肠道副溶血性弧菌载量,改善结肠炎性细胞浸润和胶原沉积症状,增加肠上皮杯状细胞数(P<0.05);高剂量JZXJ-7还可以显著抑制细胞因子介导的过度炎症,提高抗氧化酶活力,改善肠壁通透性;此外,JZXJ-7能够显著增加肠道微生物的多样性,部分逆转菌群紊乱现象,富集阿克曼菌属、双歧杆菌属和梭菌属等有益菌群,重塑肠道微生态稳定。综上,JZXJ-7可有效缓解副溶血性弧菌感染小鼠的结肠病理损伤,维系肠道功能,其作用机制与对肠道菌群的调控作用有关。The protective effect of Bacillus subtilis JZXJ-7(JZXJ-7)on Vibrio parahaemolyticus(Vp)infected mice was investigated.Totally 28 male C57BL/6J mice were randomly divided into four groups:a Vp infection model group(infected with the standard virulence strain ATCC33847),two JZXJ-7 intervention groups(administered at low(10^(6) CFU)and high(10^(8) CFU)doses by gavage),and a control group.The control and model groups were administered with an equal volume of normal saline.Disease activity index(DAI),Vp load in the intestine tract(including the ileum,cecum,and colon),colonic pathological alterations,colonic cytokine contents,oxidative stress resistance,and changes in intestinal microbial community composition were detected in all groups.The results showed that the protective effect of JZXJ-7 was better at the high dose than at the low dose.High-dose JZXJ-7 significantly reduced the DAI and intestinal Vp load in Vp infected mice,alleviated inflammatory cell infiltration and collagen deposition,and increased the number of intestinal epithelial goblet cells(P<0.05).Moreover,high-dose JZXJ-7 significantly inhibited cytokine-mediated inflammation,increased antioxidant enzyme activities and enhanced intestinal permeability. Furthermore, JZXJ-7 significantly increased (P < 0.05) intestinal microbial diversity, partially reversing intestinal flora disturbance, enriching Akkermansia, Bifidobacterium, Clostridium and other beneficial bacteria, and thus resulting in the restoration of intestinal microecological homeostasis. In summary, JZXJ-7 can effectively alleviate colonic pathological damage in Vp infected mice and maintain intestinal functions, and the underlying mechanism may be related to its regulatory effect on the intestinal microbiota.

关 键 词：枯草芽孢杆菌JZXJ-7 副溶血性弧菌 感染 肠道微生态 保护作用 

分 类 号：TS201.3[轻工技术与工程—食品科学]