β-胡萝卜素对染镍肺泡巨噬细胞抗氧化酶活性的影响  

Effects of β-Carotene on Activities of Antioxidant Enzyme of Ni_2O_3 Exposed Alveolar Macrophage

作　　者：张敬[1] 刘艺敏[1] 张军[2] 邱水强[3] 龙继贤[4] 蔡亚滨[3] 

机构地区：[1]同济大学医学院预防医学教研室,上海200070 [2]同济大学医学院生物教研室,上海200331 [3]同济大学医学院98级学生,上海200072 [4]3同济大学医学院98级学生,上海200072

出　　处：《工业卫生与职业病》2002年第6期325-327,共3页Industrial Health and Occupational Diseases

基　　金：铁道部科技基金资助项目 (J2 0 0 0Z10 8)

摘　　要：目的　研究 β -胡萝卜素拮抗三氧化二镍的细胞毒作用。 方法　采用体外细胞培养法测定在β-胡萝卜素(2 5 ,5 0 ,10 0 μmol/L)作用下 ,体外染镍肺泡巨噬细胞 (AM)的存活率及活力。同时观察细胞内活性氧的产生 ,以及过氧化氢酶 (CAT)和谷胱甘肽过氧化物酶 (GSH Px)活力的变化。结果　在体外染镍肺泡巨噬细胞中加入不同浓度的 β -胡萝卜素后可提高该细胞的存活率和活力 ,可增强细胞内CAT、GSH Px的活性 ,其中以 10 0 μmol/L的 β -胡萝卜素最为明显。 结论　镍可致细胞脂质过氧化 ,β -胡萝卜素具有拮抗镍对肺泡巨噬细胞的细胞毒作用 ,可能与抗氧化损伤有关。Objective To study the antagonism of β\|carotene on cytotoxic effect by Ni 2O 3.Methods Ni 2O 3 exposed alveolar macrophages were cultured in vitro with 25,50,and 100 μmol/L β\|carotene added respectively and their survival rates and activities were measured.In addition,the production of active oxygen,activity changes of catalase(CAT)and glutathione peroxidase(GSH Px)were observed.Results It revealed that β\|carotene elevated survival rates and activities of Ni 2O 3 exposed alveolar macrophages and enhanced the activities of CAT and GSH Px,especially when β\|carotene at 100 μmol/L .Conclusions β\|carotene could antagonize lipid peroxidation of alveolar macrophage induced by Ni 2O 3,which is probably due to its antioxidant property.

关 键 词：肺泡巨噬细胞 Β-胡萝卜素 过氧化氢酶 谷胱甘肽过氧化物酶 肺癌 致癌机制 

分 类 号：R734.2[医药卫生—肿瘤]