抗噬菌体菌株A46利用淀粉糖发酵2-KG的研究  

Study on Fermentation Conditions of 2-Keto-D-Gluconic Acid Using Glucose as Carbon Source by Phage-Resistant Mutant A46

作　　者：赵峰梅[1] 崔凤霞 王咏梅 米丽娟[1] 孙文敬[1] 蒋明珠[1] 

机构地区：[1]山西省生物研究所,山西太原030006 [2]郑州市生物化工厂,河南郑州450007

出　　处：《山西农业科学》2002年第4期68-71,共4页Journal of Shanxi Agricultural Sciences

摘　　要：抗噬菌体菌株A4 6可以利用淀粉水解糖代替结晶D -葡萄糖进行 2 -酮基 -D -葡萄糖酸( 2 -KG)的发酵。试验结果表明 ,用淀粉水解糖作碳源进行 2 -KG发酵的条件为 :接种量 8% ,发酵摇瓶装量 2 0ml,培养温度 31℃ ,发酵周期 72h ;发酵最佳配比为 :淀粉水解糖 16 .5% ,玉米浆 1.6 5% ,尿素 0 .0 0 3% ,KH2 PO4 0 .0 0 15%。Glucose obtained by starch degradation can be used as carbon source instead of crystal glucose in the fermentation of 2-keto-D-gluconic acid by phage resistant mutant A46. The results showed the optimal fermentation conditions were the following: the inoculation amount was 8%, the volume of medium in shaking flask 20ml, temperature 31℃ and fermentation period 72h. In addition, the optimal ratio of medium are: glucose 16.5%, corn steep liquor 1.65%, urea 0.003% and KH 2PO 4 0.0015%.

关 键 词：抗噬菌体 菌株A46 淀粉糖 发酵 2-KG 淀粉水解糖 

分 类 号：Q936[生物学—微生物学]