棕榈酸虾青素酯的合成分离及抗氧化活性分析  

Synthesis,isolation and antioxidant activity of astaxanthin palmitate

作　　者：王长露 张颖 翁文 WANG Changlu;ZHANG Ying;WENG Wen(College of Chemistry,Engineering and Environment,Minnan Normal University,Zhangzhou 363000,China;Zhangzhou City Vocational College,Zhangzhou 363000,China)

机构地区：[1]闽南师范大学化学化工与环境学院,福建漳州363000 [2]漳州城市职业学院,福建漳州363000

出　　处：《应用化工》2024年第11期2662-2666,2671,共6页Applied Chemical Industry

基　　金：福建省科技厅引导性项目资助(2020Y0054)。

摘　　要：以虾青素与棕榈酸作为反应底物酯化合成棕榈酸虾青素双酯,优化出最佳反应条件为:虾青素50 mg、棕榈酸45 mg、二氯甲烷1 mL、1-乙基-3(3-二甲基丙胺)碳二亚胺(EDCI)80 mg和4-二甲氨基吡啶(DMAP)40 mg,氮气保护下25℃避光反应12 h。通过柱层析纯化获得棕榈酸虾青素双酯和棕榈酸虾青素单酯,纯度分别为98.9%和97.4%,利用红外、核磁、高分辨质谱等对产品进行了结构表征。抗氧化实验表明,抗氧化效果总体为棕榈酸虾青素单酯>虾青素>棕榈酸虾青素双酯。Astaxanthin palmitate diester was synthesized by esterification of astaxanthin and palmitic acid,and the optimum reaction conditions were as follows:50 mg of astaxanthin,45 mg of palmitic acid,1 mL of dichloromethane,80 mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 40 mg of 4-dimethylaminopyridine,under nitrogen protection at 25℃for 12 h.Astaxanthin palmitate diester and astaxanthin palmitate monoester were purified by column chromatography with purities of 98.9%and 97.4%,respectively.The structure of the product was characterized by IR,NMR and high-resolution mass spectrometry.The antioxidant experiments showed that the overall antioxidant effect was astaxanthin palmitate monoester>astaxanthin>astaxanthin palmitate diester.

关 键 词：虾青素 棕榈酸 棕榈酸虾青素单酯 棕榈酸虾青素双酯 抗氧化活性 

分 类 号：TQ413.23[化学工程] TS209[轻工技术与工程—食品科学]