LC-MS/MS和GICA检测小麦中呕吐毒素质量分数及分布规律  

Determination of Mass Fraction and Distribution Pattern of Deoxynivalenol in Wheat by LC-MS/MS and GICA

作　　者：李小芳 张嘉 刘丽强[1] 李丁[1] 匡华[1] 胥传来[1] 郭玲玲 LI Xiaofang;ZHANG Jia;LIU Liqiang;LI Ding;KUANG Hua;XU Chuanlai;GUO Lingling(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2024年第11期90-97,共8页Journal of Food Science and Biotechnology

基　　金：国家“十四五”重点研发计划项目(2020YFC1606804)。

摘　　要：脱氧雪腐镰刀菌烯醇(deoxynivalenol, DON)通常被称为呕吐毒素,主要由禾谷镰刀菌产生,具有神经毒性、免疫毒性、胚胎毒性、致畸性和致癌性。DON主要污染小麦,不仅会导致小麦品质下降,还会直接或间接危害人体健康。分别通过液相色谱-串联质谱(LC-MS/MS)法和胶体金免疫层析(GICA)法检测小麦中的DON质量分数,通过加标回收试验评估2种方法的准确度及稳定性,2种方法的回收率分别为92.98%~97.43%和104.53%~108.92%,且相对标准偏差(RSD)均小于10%。分别用2种方法检测天然污染小麦加工后各部分的DON质量分数。磨粉加工后,面粉与全麦粉中DON质量分数的平均比值为0.56,麸皮与全麦粉中DON质量分数的平均比值为1.55。结果表明,小麦经磨粉加工后,面粉中DON质量分数与全麦粉相比显著降低,而麸皮中DON质量分数与全麦粉相比显著升高,因此小麦加工时将麸皮最大化分离可以有效降低面粉中DON质量分数。Deoxynivalenol(DON),also known as vomitoxin,is produced by Fusarium graminearum.It is neurotoxic,immunotoxic,embryotoxic,teratogenic,and carcinogenic.DON mainly contaminates wheat,which not only leads to the quality deterioration of wheat but also jeopardizes the health of human beings directly or indirectly.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)and gold immunochromatographic assay(GICA)were employed to determine the mass fraction of DON in wheat.The accuracy and stability of the two methods were evaluated by the spiked recovery test,and the recovery of the two methods was 92.98%~97.43%and 104.53%~108.92%,respectively,with the relative standard deviations(RSD)less than 10%.Then,the two methods were used to determine the mass fraction of DON in each part of naturally polluted wheat after processing.After milling and processing,the mass fraction ratio of DON in flour to whole wheat flour averaged 0.56,and that of DON in bran to whole wheat flour averaged 1.55.The results showed that the mass fraction of DON in flour was significantly lower than that in whole wheat flour after milling and processing of wheat,while the mass fraction of DON in bran was significantly higher than that in whole wheat flour.Therefore,the separation of bran from wheat after processing to the maximal extent can effectively reduce the mass fraction of DON in flour.

关 键 词：呕吐毒素 小麦磨粉加工 分布规律 液相色谱-串联质谱 胶体金试纸条 胶体金免疫层析 

分 类 号：TS2[轻工技术与工程—食品科学与工程]