H_(2)O_(2)制备皂角米多糖及其结构表征和抗氧化活性研究  

Preparation of polysaccharides from Gleditsia sinensis Lam.seed using H_(2)O_(2)and its structural characterization and antioxidant activity

作　　者：吴雯雯 蒲龙林 朱德全 邹大江[1,2] 姚秋萍 WU Wen-wen;PU Long-lin;ZHU De-quan;ZOU Da-jiang;YAO Qiu-ping(School of Ethnic Medicine,Guizhou Minzu University,Guiyang 510025,Guizhou,China;Resources Development and Utilization State Ethnic Affairs Commission Key Laboratory of Ethnic Guizhou Minority Medical,Guiyang 510025,Guizhou,China)

机构地区：[1]贵州民族大学民族医药学院,贵州贵阳510025 [2]贵州少数民族医药资源开发与利用国家民委重点实验室,贵州贵阳510025

出　　处：《粮食与油脂》2025年第2期139-145,151,共8页Cereals & Oils

基　　金：国家自然科学基金(32260585);贵州省科技计划项目(黔科合支撑[2021]一般136);贵州省林业基础研究项目(黔林科合[2021]09)。

摘　　要：通过响应面法优化H_(2)O_(2)制备皂角米多糖的工艺,并对产物结构进行表征,探讨其抗氧化能力。结果表明:H_(2)O_(2)制备皂角米多糖的最佳条件为H_(2)O_(2)体积分数3.0%、提取温度80℃、提取时间7 h,在此条件下皂角米多糖得率为36.30%±0.76%,重均分子量为162 kDa。皂角米多糖由甘露糖、葡萄糖和半乳糖组成,其中甘露糖与半乳糖含量比值为2.14。制得的皂角米多糖主要由t-Galp、t-Manp、1,4-Manp、1,6-Glcp、1,4-Glcp、1,4,6-Galp组成,含量分别为5.49%、20.60%、43.06%、1.25%、2.79%、26.80%。当H_(2)O_(2)提取的皂角米多糖质量浓度为5.00 mg/mL时,其对DPPH·、·O-2的清除率以及还原力分别为87.40%±1.89%、83.28%±1.96%和0.723±0.027。The preparation process of Gleditsia sinensis Lam.seed polysaccharides using H_(2)O_(2)was optimized by response surface methodology,the product structure was characterized,and its antioxidant capacity was explored.The results showed that the optimal conditions for preparing Gleditsia sinensis Lam.seed polysaccharides with H_(2)O_(2)were H_(2)O_(2)volume fraction 3.0%,extraction temperature 80℃,and extraction time 7 h.Under these conditions,the yield of Gleditsia sinensis Lam.seed polysaccharides was 36.30%±0.76%,and the weight-average molecular weight was 162 kDa.Gleditsia sinensis Lam.seed polysaccharides were composed of mannose,glucose and galactose,with a ratio of 2.14 between mannose and galactose content.The Gleditsia sinensis Lam.seed polysaccharides were mainly composed of t-Galp,t-Manp,1,4-Mnp,1,6-Glcp,1,4-Glcp,and 1,4,6-Galp,with contents of 5.49%,20.60%,43.06%,1.25%,2.79%and 26.80%,respectively.When the mass concentration of Gleditsia sinensis Lam.seed polysaccharide extracted by H_(2)O_(2)was 5.00 mg/mL,its scavenging rate for DPPH·and·O_(2)^(-)and reducing power were 87.40%±1.89%,83.28%±1.96%,and 0.723±0.027,respectively.

关 键 词：皂角米 多糖 H_(2)O_(2) 结构表征 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]