黄曲霉转录因子AfsclB生物学功能分析  

The Biological Function Analysis of Aspergillus Flavus Transcription Factor AfsclB

作　　者：潘佳琦 刘一冰 王刚 邢福国[2] 李二峰 PAN Jiaqi;LIU Yibing;WANG Gang;XING Fuguo;LI Erfeng(Horticulture and Landscape College,Tianjin Agricultural University,Tianjin 300392;Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193)

机构地区：[1]天津农学院园艺园林学院,天津300392 [2]中国农业科学院农产品加工研究所,北京100193

出　　处：《核农学报》2025年第3期449-459,共11页Journal of Nuclear Agricultural Sciences

基　　金：中国农业科学院重大科技任务粮食产能持续提升战略研究(CAAS-ZDRW202419)。

摘　　要：黄曲霉(Aspergillus flavus)是一种常见的腐生型真菌,其产生的黄曲霉毒素(AFT)具有极强的致癌性,对粮食、油料和饲料等能造成严重危害。为了研究黄曲霉生长发育的调控机制,本研究通过敲除黄曲霉的转录因子基因AfsclB,探究AfsclB对黄曲霉生长发育和次级代谢的调控作用。结果表明,AfsclB缺失可显著抑制黄曲霉分生孢子形成,促进AFT生物合成。进一步通过转录组分析发现,AfsclB显著影响AFT生物合成、戊糖和葡萄糖醛酸相互转化、乙醛酸和二羧酸代谢、类固醇生物合成等代谢通路。AfsclB基因敲除后,AFT生物合成途径中的多个关键基因均上调表达,从而导致了黄曲霉毒素B1(AFB1)产量提高。因此,AfsclB基因的敲除引发了黄曲霉代谢途径的调整,影响黄曲霉的产孢能力,导致了AFB1产量增加,该基因可能是AFT合成通路的负调控因子。综上,AfsclB具有开发为分子防控靶标的潜力,本研究结果为黄曲霉及其毒素防控提供了依据。Aspergillus flavus is a prevalent saprophytic fungus known for its production of aflatoxins(AFTs),which are highly carcinogenic and pose serious threats to crops,oilseeds,and animal feeds.To investigate the regulatory mechanisms underlying the growth and development of A.flavus,this study focused on the transcription factor AfsclB by conducting a knockout experiment.The results indicated that the deletion of AfsclB significantly inhibited the formation of conidia while promoting AFT biosynthesis.Further transcriptomic analysis revealed that AfsclB notably affects metabolic pathways related to AFT biosynthesis,the interconversions of pentose and glucuronate,glyoxylate and dicarboxylate metabolism,and steroid biosynthesis.Following the knockout of AfsclB,several key genes in the AFT biosynthesis pathway were upregulated,leading to an increased production of aflatoxin B1(AFB1).Consequently,the knockout of the AfsclB gene triggered adjustments in the metabolic pathways of A.flavus,affecting its sporulation capacity and resulting in an elevated yield of AFB1.This gene may serve as a negative regulatory factor in the AFT synthesis pathway.In summary,AfsclB has the potential to be applied as a molecular control target.The results of this study provide evidence supporting the control of A.flavus and its toxin production.

关 键 词：黄曲霉 AfsclB 产毒 转录组分析 

分 类 号：TS201.6[轻工技术与工程—食品科学]