响应面法优化斜链虫草菌丝体多糖的液体深层发酵条件  

Optimization of Liquid Submerged Fermentation Condition of Mycelial Polysaccharide from Cordyceps cateniobliqua by Response Surface Methodology

作　　者：潘其贵 刘如明 邹雨佳 杨旭 梁建东 肖建辉 PAN Qigui;LIU Ruming;ZOU Yujia;YANG Xu;LIANG Jiandong;XIAO Jianhui(Institute of Medicinal Biotechnology of The Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China;Center for Mycomedicine Research,School of Basic Medicine of Guizhou University of Traditional Chinese Medicine,Guiyang 550000,China)

机构地区：[1]遵义医科大学附属医院医药生物技术研究所,贵州遵义563000 [2]贵州中医药大学基础医学院/菌物药研究中心,贵州贵阳550000

出　　处：《食品科技》2024年第12期10-18,共9页Food Science and Technology

基　　金：贵州省高层次创新型人才培养计划项目(黔科合平台人才-GCC[2022]001-1);贵州省教育厅中央财政支持地方高校改革发展专项资金项目(黔教技[2023]020号)。

摘　　要：为提高斜链虫草(Cordyceps cateniobliqua(Z.Q.Liang)Kepler,B.Shrestha&Spatafora)MB11菌丝体多糖的产量,对斜链虫草的液体深层发酵条件进行优化。采用单因素试验优化了斜链虫草菌丝体多糖发酵的培养基成分,在此基础上,运用Plackett-Burman试验、最陡爬坡试验、Box-Behnken响应面法进一步优化了培养基、接种量、装液量、温度和初始pH值等发酵生产条件。优化后的培养条件如下:蔗糖30 g/L、黄豆匀浆滤液6.84 g/L、MgSO_(4)·7H_(2)O 0.93 g/L、KCl 1 g/L、维生素C 1 g/L、接种量4%、装液量50%、发酵温度18.0℃、摇床转数160 r/min、初始pH6.25。在最优条件下,斜链虫草菌丝体多糖的产量为(703.31±19.93)mg/mL,比优化前提高了3.76倍。In order to enhance the production of mycelial polysaccharides of Cordyceps cateniobliqua(Z.Q.Liang)Kepler,B.Shrestha&Spatafora MB11,its production conditions of Cordyceps cateniobliqua was optimized under submerged liquid fermentation.The medium compositions including carbon and nitrogen sources,inorganic salts,and growth factors were screened by single-factor experiment method.Based on the result of single factor experiment,Plackett-Burman test,steepest ascent test,and Box-Behnken response surface method were successively used to optimize the production condition of mycelial polysaccharides.such as medium,liquid amount,inoculum size,fermentation temperature,and initial pH value.The optimal fermentation condition was obtained as follows:sucrose 30 g/L,soybean homogenate filtrate 6.84 g/L,MgSO_(4)·7H_(2)O 0.93 g/L,KCl 1 g/L,vitamin C 1 g/L,inoculum concentrations 4%,liquid volume 50%,fermented temperature 18.0℃,rotational speed 160 r/min,initial pH 6.25.Under the optimal fermentation condition,the yield of mycelial polysaccharides of Cordyceps cateniobliqua was up to(703.31±19.93)mg/mL,which was 3.76-fold higher than prior to optimization.

关 键 词：斜链虫草 PLACKETT-BURMAN 最陡爬坡试验 响应面优化 菌丝体多糖 液体深层发酵 

分 类 号：TS201.3[轻工技术与工程—食品科学]