低聚半乳糖结构鉴定及体外抗炎活性分析  

Structural Identification of Galacto-Oligosaccharides and Analysis of Anti-Inflammatory Activity in Vitro

作　　者：刘彤 郭玉娟 刘思琪 那治国[1,2] LIU Tong;GUO Yujuan;LIU Siqi;NA Zhiguo(College of Food Engineering,Harbin University of Commerce,Harbin 150028,China;School of Food Engineering,East University of Heilongjiang,Harbin 150066,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028 [2]黑龙江东方学院食品工程学院,黑龙江哈尔滨150066

出　　处：《食品科技》2024年第12期249-257,共9页Food Science and Technology

基　　金：哈尔滨商业大学博士科研支持计划项目(22BQ66)。

摘　　要：通过β-半乳糖苷酶制备低聚半乳糖(Galacto-oligosaccharide,GOS),并探究GOS的抗炎活性。将牛乳水解液进行分离纯化及结构表征,最后通过细胞实验研究其体外抗炎活性。结果表明:优化获得的最佳分离条件为10 mL乳液,使用去离子水作为流动相,流速为20 mL/h,每隔2 mL收集一管。通过高效液相色谱-串联质谱(High performance liquid chromatography-mass spectrometry,HPLC-MS)分析确认,所得化合物的分子式分别为C_(18)H_(32)O_(16)与C_(24)H_(42)O_(21),分别被鉴定为低聚半乳三糖(tri-GOS)和低聚半乳四糖(tetra-GOS)。与脂多糖组相比,tri-GOS和tetra-GOS能显著降低一氧化氮(Nitric oxide,NO)的产生,肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)的分泌受到抑制,抑制率分别为47.71%、57.75%;二者均能抑制白介素-6(Interleukin-6,IL-6),抑制率分别为36.91%、46.31%。这说明GOS具有较好的抗炎活性,其中tetra-GOS效果优于tri-GOS。采用β-半乳糖苷酶水解牛乳可获得具有显著抗炎功能的tri-GOS、tetra-GOS,可为开发具有抗炎功能的低乳糖牛乳提供理论及技术支持。Galacto-oligosaccharides(GOS)were prepared byβ-galactosidase hydrolysis and investigated the anti-inflammatory activity.The milk hydrolysate was isolated,purified and characterized,and its anti-inflammatory activity in vitro was studied by cell experiments.The results showed that the optimal separation conditions were as follows:10 mL milk,deionized water as the mobile phase,a flow rate of 20 mL/h,and one tube collected every 2 mL.By high performance liquid chromatography-mass spectrometry(HPLC-MS)analysis confirmed that the molecular formula of the obtained compounds were C_(18)H_(32)O_(16)and C_(24)H_(42)O_(21),respectively,which were identified as tri-GOS and tetra-GOS.Compared with lipopolysaccharide(LPS)group,tri-GOS and tetra-GOS significantly decreased the production of nitric oxide(NO).The secretion of tumor necrosis factor-α(TNF-α)was inhibited,and the inhibition rates were 47.71%and 57.75%,respectively.The inhibition rates of interleukin-6(IL-6)were 36.91%and 46.31%,respectively.These results indicate that GOS has good anti-inflammatory activity,tetra-GOS has the strongest anti-inflammatory activity compared with tri-GOS.β-galactosidase can be used to hydrolyze milk to obtain tri-GOS and tetra-GOS with significant anti-inflammatory function,providing theoretical and technical support for the development of low-lactose milk with specific anti-inflammatory functions.

关 键 词：低聚半乳糖 酶水解 结构鉴定 抗炎活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]