草鱼骨胶原多肽的制备工艺优化及抗氧化活性研究  

Optimization of preparation process of collagen polypeptide from grass carp bones and its antioxidant activity

作　　者：张逸轩 黄诗姝 曹健明 杨正 黄晓苹 ZHANG Yixuan;HUANG Shishu;CAO Jianming;YANG Zheng;HUANG Xiaoping(College of Food Science and Technology,Wuhan Business University,Wuhan 430056)

机构地区：[1]武汉商学院食品科技学院,武汉430056

出　　处：《中国食品添加剂》2025年第1期76-83,共8页China Food Additives

基　　金：武汉商学院大学生创新创业训练计划项目(202311654149);武汉商学院博士基金项目(X2023051508562045)。

摘　　要：以草鱼骨为原材料,通过蛋白酶酶解法制备较高抗氧化活性的胶原多肽。对草鱼骨进行成分分析;以胶原多肽得率为评价指标,从风味蛋白酶、中性蛋白酶和碱性蛋白酶中筛选最优酶,并通过单因素实验和正交试验确定草鱼骨胶原多肽的最佳制备工艺;以DPPH·、O_(2)·^(-)、·OH、ABTS^(+)·清除率和还原能力为指标测定草鱼骨胶原多肽的抗氧化活性。结果表明草鱼骨可作为一种高蛋白低脂肪(粗蛋白质含量约26.35%,粗脂肪含量约9.53%)优质原料;碱性蛋白酶为最优酶,制备胶原多肽的最优工艺为:酶解温度50℃、加酶量9000 U/g底物、料液比为1∶15(w/v)、pH为8.0,此时胶原多肽得率为(54.34±0.35)%;抗氧化实验表明胶原多肽有较强的DPPH·、O_(2)·^(-)、·OH、ABTS^(+)·清除能力和还原能力。该研究为鱼骨资源的开发利用提供了技术支持和参考。Using grass carp bones as raw material,collagen polypeptide with high antioxidant activity was prepared from grass carp bones using enzymatic hydrolysis.The composition of grass carp bones was analyzed.The optimal enzyme was selected from flavor protease,neutral protease,and alkaline protease based on the yield of collagen peptides as the evaluation index.The best preparation process for collagen peptides from grass carp bones was determined through single-factor experiments and orthogonal tests.The antioxidant activity of collagen polypeptide was measured by DPPH·,O_(2)^(-),·OH,ABTS^(+)·scavenging capacity,and reducing ability.The results indicated that grass carp bone could be used as a high-quality raw material with high protein and low fat(protein:26.35%,fat:9.53%).Alkaline protease was identified as the optimal enzyme,and response surface methodology was employed to determine the optimal enzymatic hydrolysis conditions:hydrolysis temperature of 50℃,enzyme amount of 9000 u/g substrate,solid-liquid ratio of 1∶15,and hydrolysis pH of 8.0.Under the above conditions,collagen polypeptide yield reached(54.34±0.35)%.The collagen polypeptide exhibited strong antioxidant activity,effectively scavenging DPPH·,O_(2)·^(-),·OH,ABTS^(+)·radicals and demonstrated strong reducing power.This study provides technical support and reference for the development and utilization of fish bone resources.

关 键 词：草鱼骨 胶原多肽 工艺优化 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]