高效液相色谱-紫外检测器法测定食品中低聚木糖含量  

Determination of xylo-oligosaccharides content in food by high performance liquid chromatography-ultraviolet detector

作　　者：尹彦洋 林枫翔 张慧 单万亭 秦琦 YIN Yan-Yang;LIN Feng-Xiang;ZHANG Hui;SHAN Wan-Ting;QIN Qi(Harbin Meihua Biotechnology Co.,Ltd.,Harbin 150010,China;Heilongjiang Drug Audit and Inspection Center,Harbin 150001,China;Heilongjiang Institute for Drug Control,Harbin 150088,China)

机构地区：[1]哈尔滨美华生物技术股份有限公司,哈尔滨150010 [2]黑龙江省药品审核查验中心,哈尔滨150001 [3]黑龙江省药品检验研究院,哈尔滨150088

出　　处：《食品安全质量检测学报》2025年第4期272-278,共7页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱-紫外检测器法测定食品中低聚木糖含量的方法。方法前处理方法中考察称样量,水解时间,衍生时间,净化次数;色谱条件对梯度洗脱和等度洗脱进行了比较,同时考察了柱温和检测波长。结果最佳的称样量为2.0 g,最佳的水解时间为100 min,最佳的衍生时间为100 min,最佳净化次数为3次,流动相采取梯度洗脱方法最佳,最佳的柱温为30℃,最佳的检测波长为250 nm。结论该方法测定食品中低聚木糖具有良好的准确性和灵敏度,本研究为食品中开发低聚木糖含量测定方法提供数据支持。Objective To establish a high performance liquid chromatography-ultraviolet detector method for the determination of xylo-oligosaccharide content in food.Methods The sample size,hydrolysis time,derivatization time and purification times in the pre-treatment method were investigated.Gradient elution was compared with isocratic elution to fix the chromatographic conditions.Moreover,the detection wavelength and column temperature were obtained at the same time.Results The optimized condition was as follows:Weighing sample 2.0 g;hydrolysis time 100 min;derivation time 100 min;purification for 3 times;mobile gradient elution was employed,column temperature 30℃;detection wavelength 250 nm.Conclusion This method has good accuracy and sensitivity for the determination of xylo-oligosaccharides in food.This study provides data support for the development of a method for the determination of xylo-oligosaccharides in food.

关 键 词：高效液相色谱-紫外检测器法 低聚木糖 前处理方法 色谱条件 

分 类 号：R28[医药卫生—中药学]