基于代谢组学探究饮酒对大鼠血脂影响及机制  

Effects and mechanisms of drinking on blood lipid in rats based on metabolomics

作　　者：丁君平 宋艳 黄张君 余松柏 刘蓉[1] 曾南[1] DING Junping;SONG Yan;HUANG Zhangjun;YU Songbai;LIU Rong;ZENG Nan(State Key Laboratory of Southwest Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Luzhou Pinchuang Technology Co.,Ltd.,Luzhou 646000,China)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137 [2]泸州品创科技有限公司,四川泸州646000

出　　处：《中国酿造》2025年第2期32-40,共9页China Brewing

基　　金：国家固态酿造工程技术研究中心资助校企合作项目(2021HX01)。

摘　　要：采用超高效液相色谱-四级杆静电场轨道阱高分辨率质谱(UPLC-Q/Orbitrap-HRMS)非靶向代谢组学方法,选择SD大鼠为研究对象,按照推荐成人最大饮酒量(25 g/d),分别给予低剂量(2.63 mL/kg)和高剂量(5.25 mL/kg)的某品牌52°浓香型白酒及体积分数52%乙醇(5.25 mL/kg),持续30 d,探究不同给酒剂量对SD大鼠脂质代谢物以及脂质代谢相关通路变化的影响。结果表明,高剂量及低剂量连续给酒30 d,对大鼠体质量、肝脏指数、肝功能及血脂功能指标均无显著影响(P>0.05);体积分数52%乙醇灌胃30 d,可引起大鼠脂代谢紊乱,总胆固醇(TC)水平显著升高(P<0.05)。基于血清脂代谢组分的正交偏最小二乘-判别分析(OPLS-DA)能有效区分各实验组,共鉴定出814种脂质,以甘油三酯类和磷脂酰胆碱类物质为主。随着饮酒剂量的加大,大鼠发生变化的脂质代谢物数量越多。饮酒对脂质代谢相关通路主要富集于甘油磷脂代谢、亚油酸和α-亚麻酸代谢通路,乙醇组脂质代谢物还富集到胰岛素抵抗通路和脂质与动脉粥样硬化通路。SD rats were selected as research subjects and were administered low-dose(2.63 ml/kg)and high-dose(5.25 ml/kg)of a certain brand of 52°strong-flavor(Nongxiangxing)Baijiu,as well as ethanol with volume fraction 52%(5.25 ml/kg)for 30 d,according to the recommended maximum daily alcohol intake for adults(25 g/d).The effects of different doses of Baijiu on lipid metabolites and lipid metabolism related pathways in SD rats were explored by non-targeted metabolomics method of ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution-mass spectrometry(UPLC-Q/Orbitrap-HRMS).The results shown that continuous administration of low-and high-doses of Baijiu for 30 d had no significant effects on the body mass,liver index,liver function and blood lipid function indexes of rats(P>0.05).However,after 30 d of intragastric administration of ethanol with volume fraction 52%,the lipid metabolism dysregulation was induced in the rats,with a significant increased in total cholesterol(TC)levels(P<0.05).The experimental groups could be effectively distinguished by orthogonal partial least squares-discriminant analysis(OPLS-DA)based on blood lipid metabolites,and a total of 814 lipids were identified,predominantly triglycerides and phosphatidylcholines.As the Baijiu dose increased,the number of altered lipid metabolites in the rats increased.Drinking mainly affected lipid metabolism-related pathways,which were primarily enriched in glycerophospholipid metabolism,linoleic acid and alpha-linolenic acid metabolism pathways.The lipid metabolites in the ethanol group were also enriched in insulin resistance pathway and lipid-atherosclerosis pathway.

关 键 词：浓香型白酒 合理饮酒 血脂 肝功能 脂质代谢组学 

分 类 号：TS262.3[轻工技术与工程—发酵工程] R361[轻工技术与工程—食品科学与工程]