耐高温产香酵母的分离筛选及麸曲制作工艺优化  

Isolation and screening of high-temperature resistant aroma-producing yeast and optimization of production process of Fuqu

作　　者：张旭姣 闫裕峰 郎繁繁 王晨苑 郑宇 ZHANG Xujiao;YAN Yufeng;LANG Fanfan;WANG Chenyuan;ZHENG Yu(Shanxi Zilin Vinegar Co.,Ltd.,Taiyuan 030400,China;Key Laboratory of Vinegar Fermentation Science and Engineering of Shanxi Province,Taiyuan 030400,China;Key Laboratory of Special Sorghum Breeding and Processing,Ministry of Agriculture and Rural Areas,Taiyuan 030400,China;School of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]山西紫林醋业股份有限公司,山西太原030400 [2]食醋发酵科学与工程山西省重点实验室,山西太原030400 [3]农业农村部特种高粱育种及加工重点实验室,山西太原030400 [4]天津科技大学生物工程学院,天津300457

出　　处：《中国酿造》2025年第2期108-114,共7页China Brewing

基　　金：山西省重点研发计划(202102130501008,202202140601018,202202130501011);山西省科技厅山西省重点实验室建设项目(202204010931002);山西省农业关键核心技术攻关项目(NYGG15-3)。

摘　　要：为提升食醋香气成分含量,该研究经过透明圈法初筛,耐高温、耐乙醇复筛,从山西老陈醋酿造过程取样筛选耐高温产香酵母。通过形态学观察、生理生化试验及分子生物学技术对筛选菌株进行鉴定。将所筛选菌株制作成酵母麸曲,通过单因素及正交试验对麸曲制作工艺进行优化,并应用于高粱汁发酵,采用顶空固相微萃取(HS-SPME)结合气相质谱(GC-MS)分析其挥发性风味物质。结果表明,筛选出一株耐高温耐乙醇产香酵母菌株ZLCY-93,经鉴定为扣囊复膜酵母(Saccharomycopsis fibuligera)。菌株ZLCY-93麸曲最适培养条件为粮水比1.0∶1.0(g∶mL),接种量5%,培养温度28℃,培养时间28 h。在此优化条件下,ZLCY-93麸曲的酵母数可达1.72×109 CFU/g。将该麸曲应用于高粱汁发酵,共检出挥发性风味物质34种,其中,酯类23种、醇类7种,醛类、酮类、酸类、肟类各1种。酯类及醇类物质相对含量为69.68%,表明该产香麸曲具有良好的产香性能。In order to improve the content of aroma components in vinegar,a high-temperature resistant aroma-producing yeast was preliminary screened by transparent ring method and re-screened by high-temperature and ethanol resistance tests from the brewing process of Shanxi aged vinegar.The screened strain was identified by morphological observation,physiological and biochemical experiments and molecular biological technique.The screened strain was made into yeast Fuqu and the production technology of Fuqu was optimized by single factor and orthogonal experiments,and applied to sorghum juice fermentation.The volatile flavor substances were analyzed by headspace solid-phase microextraction(HS-SPME)combined with gas chromatography-mass spectrometry(GC-MS).The results showed that one aromatic strain ZLCY-93 with high temperature and ethanol tolerance was screened and identified as Saccharomycopsis fibuligera.The optimal culture conditions of strain ZLCY-93 were as follows:grain and water ratio 1.0∶1.0(g∶ml),inoculum 5%,culture temperature 28℃,and culture time 28 h.Under the optimal conditions,the yeast number of ZLCY-93 Fuqu could reach 1.72×109 CFU/g.The optimized ZLCY93-Fuqu was applied to sorghum juice fermentation,and a total of 34 volatile flavor substances were detected,including 23 esters,7 alcohols,1 aldehyde,1 ketone,1 acid and 1 oxime.The relative content of esters and alcohols was 69.68%,which indicated that the aroma-producing Fuqu had good aroma-producing performance.

关 键 词：扣囊复膜酵母 分离筛选 酵母麸曲 制曲工艺优化 挥发性风味物质 

分 类 号：TS261.1[轻工技术与工程—发酵工程]