Arctiin Protects Chondrocytes from Interleukin-1β-Induced Inflammation and Apoptosis by RNA Sequence In vivo and In vitro  

作　　者：Yong-Jia Song Jia-Min Bao Zeng-Qiao Zhang Yun-Xiang Hai Hao-Nan Wen Tian-Jun Zhai Wei Feng Min Song 

机构地区：[1]Clinical College of Chinese Medicine,Gansu University of Chinese Medicine,Lanzhou,Gansu,China [2]School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai,China [3]Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai,China

出　　处：《World Journal of Traditional Chinese Medicine》2024年第4期495-511,共17页世界中医药杂志(英文)

基　　金：supported by the National Natural Science Foundation of China (81873328)

摘　　要：Objective:Osteoarthritis(OA) is a progressive joint disease characterized by degeneration and de struction of articular cartilage.Arctiin(ARC) has been shown in many studies to have potential anti-inflammatory,anti-apoptotic,and antioxidant effects in various diseases.However,the mechanism by which ARC exerts its protective effects in OA is not fully understood.Here,we explore the mechanism by which ARC plays its protective role in OA.Materials and Methods:Mouse chondrocytes were isolated and characterized through toluidine blue staining and collagen Ⅱ immunofluorescence labeling.A mouse-based experimental model was developed to induce chondrocyte inflammation through Interleukin-1β(IL-1β).Sub sequently,ARC was administe red in various doses to mitigate this inflammation.Techniques such as biochemical assay s,Enzyme-linked immunosorbent assay,quantitative real-time polymerase chain re action(qRT-PCR),Western blotting,and immunofluorescence labeling were employed to detect changes in nitric oxide(NO),lactate dehydrogenase(LDH),inflammatory markers,and components of the cartilage matrix in chondrocytes.RNA-sequencing(RNA-seq) was utilized to explore variations in gene expression among chondrocyte s acro ss different groups.The genes and signaling pathways that were identified underwe nt analysis through Gene Ontology and Kyoto Encyclope dia of Genes and Genomes enrichment studies.Validation of gene and protein e xpression was carried out using qRT-PCR,Western blotting,and cellular flow cytometry,based on the results from sequencing,Furthermore,Safranin-O fast green staining and immunohistochemistry staining were performed on slices of the mice knee joint to evaluate the OA Research Society International score,alterations in the cartilage matrix,and levels of apopto sis-related proteins at sites of knee cartilage damage in an arthritis model induced by mono sodium iodo acetate(MIA) and phy sical activity.Results:It was found that ARC effectively inhibits the production of IL-1β-induced chondrocytes' inducible N

关 键 词：APOPTOSIS ARCTIIN CHONDROCYTES INFLAMMATION OSTEOARTHRITIS RNA‑sequencing 

分 类 号：R285[医药卫生—中药学]