Hepatorenoprotective effects of Lepidium draba L.extracts against cyclophosphamideinduced oxidative injuries in rats via reducing apoptosis and inflammation  

作　　者：Yu-Lai You Sheng Zheng Cheng-Jian Zhao Ye-Fei Chang Pei Liu Xue-Li Zeng Lian Liu 

机构地区：[1]Department of Hepatobiliary Surgery,Chongqing University Jiangjin Hospital,Chongqing 402260,China [2]Department of Gastroenterology,The Third People's Hospital of Yunnan Province,Kunming,Yunnan 650011,China [3]Hepatobiliary Pancreatic Surgery,Qingdao Municipal Hospital,Qingdao,Shandong 266071,China [4]Department of Laboratory Medicine,The Third People's Hospital of Yunnan Province,Kunming,Yunnan 650011,China [5]Graduate School of Clinical Medicine,Dali University,Dali,Yunnan 671000,China [6]Department of Pharmacy,Shanghai Fengxian District Central Hospital,Shanghai 201406,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2025年第2期53-64,I0002,I0003,共14页亚太热带生物医学杂志(英文版)

基　　金：supported by the Basic Research Joint Special General Project of Yunnan Provincial Local Universities(part)(No:202301BA070001-029,202301BA070001-044);Yunnan Province High-level Scientific and Technological Talents and Innovation Team Selection Special Young and Middle-aged Academic and Technical Leaders Reserve Talent Project(No:202405AC350067).

摘　　要：Objective:To investigate the protective effects of Lepidium draba L.(L.draba)on cyclophosphamide(CP)-induced hepatotoxicity and nephrotoxicity in rats.Methods:A total of 36 rats were divided into six groups as follows:the sham control group,the CP group(CP 100 mg/kg i.p.on days 1,7,14,21,28,and 35),the CP groups treated with L.draba extract(100,200 and 400 mg/kg of L.draba extract for 28 d),and the L.draba extract alone group(400 mg/kg of L.draba extract for 28 d).Serum parameters of renal and hepatic function,as well as pro-inflammatory and anti-inflammatory cytokines associated with liver and kidney damage were measured.Moreover,Bax,Bcl-2,and caspase-3 gene expression and histopathological changes were assessed.Results:L.draba extract alleviated CP-induced hepatotoxicity and nephrotoxicity by decreasing nitric oxide,TBARS,IL-6,TNF-α,and IL-1βlevels,as well as increasing superoxide dismutase,catalase and glutathione peroxidase activities,and FRAP,MIF,and TGF-βlevels.In addition,the extract downregulated the expression of pro-apoptotic genes(Bax and caspase-3)and mitigated the destruction of glomeruli and renal tubules as well as the degeneration of hepatocytes.Conclusions:L.draba extract can protect hepatic and renal structure and function against CP-induced toxicities,and may be used as a therapeutic agent for CP-induced hepatotoxicity and nephrotoxicity.

关 键 词：Lepidium draba CYCLOPHOSPHAMIDE Oxidative stress Antioxidant APOPTOSIS HEPATOTOXICITY NEPHROTOXICITY 

分 类 号：R285.5[医药卫生—中药学]