一起因蜂蜜引起的钩吻碱中毒事件GC-MS快速检测和分析  

A case of honey-induced gelsemium alkaloid poisoning was rapidly detected and analyzed using GC-MS

作　　者：周海青 叶艺娟[1] 陈俏嫦 吴海军 邱丽晓 朱淇 李志娇 ZHOU Haiqing;YE Yijuan;CHEN Qiaochang;WU Haijun;QIU Lixiao;ZHU Qi;LI Zhijiao(Heyuan Center for Disease Control and Prevention,Guangdong Heyuan 517000,China)

机构地区：[1]河源市疾病预防控制中心,广东河源517000

出　　处：《中国食品卫生杂志》2024年第10期1124-1129,共6页Chinese Journal of Food Hygiene

摘　　要：目的通过对一起因蜂蜜引起的钩吻碱中毒事件快速检测和分析,为类似事件处理带来启示。方法称取或吸取适量样品和标本,加入硼砂-NaOH缓冲液(pH=9.6)摇匀后经乙酸乙酯提取,取有机层供气相色谱质谱联用仪(GC-MS)检测分析。结果蜂巢中钩吻碱含量为28.6 mg/kg,冲泡蜂蜜工具1和工具2洗脱液中钩吻碱含量分别为5.85、5.04μg,蜂蜜水中钩吻碱含量为44.0 mg/L,仅患者1~3血液中检出钩吻碱含量为22.3~402μg/L,尿液均检出钩吻碱含量为20.6~299μg/L,患者2~6尿液钩吻碱含量高于血液。结论本次食物中毒系蜂蜜含有钩吻碱,中毒剂量低,冲泡工具可以检出钩吻碱;肾脏是钩吻碱代谢途径之一,食用2 h 45 min后大部分患者尿液钩吻碱含量高于血液,应重视尿液标本采集。Objective Through the rapid detection and analysis of a case of gelsemine poisoning caused by honey,to bring inspiration for similar incident handling.Methods The appropriate amount of samples and specimen were weighed or aspirated,adding borax-NaOH buffer solution(pH=9.6),shake well,extracted with ethyl acetate,and the organic layer was used for gas chromatography-mass spectrometry detection and analysis.Results The gelsemine content in the honeycomb was 28.6 mg/kg,and the gelsemine contents in brewing honey tool 1 and tool 2 wash solution were 5.85 and 5.04μg.The gelsemine content in honey water was 44.0 mg/L,and only the blood of patients 1-3 detected gelsemine content of 22.3-402μg/L.The urine of all patients were detected gelsemine content of 20.6-299μg/L,and the urine gelsemine content of patient 2-6 was higher than that of blood.Conclusion This food poisoning was caused by honey containing gelsemine,with low poisoning dosage and which can be detected from brewing tools.The kidney is one of the metabolic pathways of gelsemine.After 2 h 45 min of consumption,most patients had higher urine gelsemine content than blood,indicating that urine specimen collection should be emphasized.

关 键 词：蜂蜜 食物中毒 钩吻碱 气相色谱质谱联用仪 生物标本 

分 类 号：R155[医药卫生—营养与食品卫生学]