食品中膳食纤维测定方法的要点与应用  

Determination of dietary fiber in foods:key points and use

作　　者：王竹 邢青斌 崔亚娟[3] 张雪松 刘玉峰[3] 王国栋 WANG Zhu;XING Qingbin;CUI Yajuan;ZHANG Xuesong;LIU Yufeng;WANG Guodong(National Institute for Nutrition and Food Safety,China Center for Disease Control and Prevention,Beijing 100050,China;Key Laboratory of Public Nutrition and Health of the National Health Commission,Beijing 100050,China;Beijing Institute of Nutrition Resources Co.,Ltd,Beijing 100069,China)

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050 [2]国家卫生健康委员会公共营养与健康重点实验室,北京100050 [3]北京市营养源研究所有限公司,北京100069

出　　处：《中国食品卫生杂志》2024年第10期1191-1198,共8页Chinese Journal of Food Hygiene

基　　金：国家重点研发项目(2022YFF0710303);国家财政项目(102393220020070000013)。

摘　　要：膳食纤维的定义包含了物质结构、生理作用和生物学效应等多重含义,故对食品中膳食纤维含量水平的精准评估需要科学的认知与严谨的测量手段。我国新版《食品安全国家标准食品中膳食纤维的测定》(GB 5009.88—2023)已经正式实施,和上一版方法相比,不仅增加了试样酶解条件,也在酶重量法基础上增加了高效液相色谱检测技术,使膳食纤维的测定结果不仅可以包括不溶性膳食纤维、可被78%乙醇沉淀的可溶性膳食纤维,也使测定不能被78%乙醇沉淀的可溶性膳食纤维成为可能,检测范围有所扩大。为了方便理解和应用,在综合国内外研究进展的基础上,本文围绕膳食纤维定义的发展和测定方法的进展,对测定方法原理、流程路径和结果表达进行了详细的解释,对方法的适用范围、与其他国家标准应用的衔接性进行了分析。The definition of dietary fiber(DF)includes multiple meanings involving material structure,physiological and biological effects.So,to accurate evaluate DF level in foods,it’s necessary to grasp scientific concepts and rigorous measurement techniques.Now,the new version of the National Food Safety Standard Determination of Dietary Fiber in Foods(GB 5009.88—2023)has been implemented in China.Compared to pre-version,high-performance liquid chromatography technique is added in enzymatic-gravimetric methods,making it possible to include insoluble and soluble dietary fiber,even that cannot be precipitated by 78%ethanol,in total dietary fiber results,and the detection scope is expanded.For better understanding the method to use,this article summaries the relation of determination methods to definition development,interprets in details about the principles,determination pathway and steps,and result expression.Simultaneously,the application scope of the method and its connection with other national standards has also been discussed.

关 键 词：膳食纤维 定义 测定方法 结果表达 

分 类 号：R155[医药卫生—营养与食品卫生学]