利用高通量测序分析冷鲜羊肉优势腐败菌群及相关抑菌剂效价评定  

Analysis of Dominant Spoilage Bacteria by High‐Throughput Sequencing and Evaluation of Inhibitory Effects of Antimicrobial Agents for Chilled Mutton

作　　者：温蕾 马艳丽 柳尧波[2] 王维婷[2] 杜鹏飞 胡鹏[2] 曹建芳 李永生[2] 贺红军[1] WEN Lei;MA Yanli;LIU Yaobo;WANG Weiting;DU Pengfei;HU Peng;CAO Jianfang;LI Yongsheng;HE Hongjun(School of Life Sciences,Yantai University,Yantai 264005,Shandong,China;Shandong Provincial Key Laboratory of Agro-Products Processing Technology,Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture and Rural Affairs,Institute of Food and Nutrition Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]山东省农业科学院农产品加工与营养研究所,山东省农产品精深加工技术重点实验室,农业农村部新食品资源加工重点实验室,山东济南250100

出　　处：《食品研究与开发》2025年第4期149-154,共6页Food Research and Development

基　　金：山东省农业科学院农业科技创新工程项目(CXGC2023F09);山东省重点研发计划项目(2022TZXD0021);国家肉羊产业技术体系建设专项(CARS-38);山东省羊产业技术体系(SDAIT-10-10);中央引导地方科技发展专项资金项目(YDZX2022149、YDZX2022135)。

摘　　要：为研究天然抑菌剂对冷鲜羊肉优势腐败菌的抑菌效力并筛选靶向抑菌剂,首先采用高通量测序法分析冷鲜羊肉在4℃贮藏过程中腐败菌的演替规律,然后针对性的选择两种醛类(柠檬醛和肉桂醛)天然抑菌剂,通过测定抑菌圈大小、最小抑菌浓度对比其对冷鲜羊肉腐败菌的抑制效果。结果表明,主要腐败菌为嗜冷假单胞菌(Pseudomonas psychrophila)、热杀索丝菌(Brochothrix thermosphacta)、溶酪葡萄球菌(Macrococcus caseolyticus)、不动杆菌(Acinetobacter)、养料嗜冷杆菌(Psychrobacter cibarius)。肉桂醛对各受试菌的抑菌能力更加显著,最小抑菌浓度为0.08~2.50 mg/mL。The inhibitory effects of natural antimicrobial agents on the dominant spoilage bacteria of chilled mutton were evaluated and targeted natural antimicrobial agents were screened.High-throughput sequencing was employed to analyze the succession pattern of spoilage bacteria of chilled mutton during storage at 4℃.Subsequently,two aldehyde-based natural antimicrobial agents(cinnamaldehyde and citral)were selected,and their inhibitory effects on spoilage bacteria were compared based on inhibition zone diameters and mini-mum inhibitory concentrations.The results indicated that the dominant spoilage bacteria were Pseudomonas psychrophila,Brochothrix thermosphacta,Macrococcus caseolyticus,Acinetobacter,and Psychrobacter cibarius.Cinnamaldehyde demonstrated more pronounced antimicrobial activities against all the tested strains,with the minimum inhibitory concentrations ranging from 0.08-2.50 mg/mL.

关 键 词：冷鲜羊肉 高通量测序 菌株鉴定 优势腐败菌 柠檬醛 肉桂醛 

分 类 号：TS2[轻工技术与工程—食品科学与工程]