核桃膳食纤维对乳杆菌属微生物体外增殖的影响  

Effect of Walnut Dietary Fiber on the Proliferation Effect of Lactobacillus in Vitro

作　　者：杨卫星 田洋[4] 岳曼[3] 盛军 徐昆龙 高晓余[1,2,3] YANG Weixing;TIAN Yang;YUE Man;SHENG Jun;XU Kunlong;GAO Xiaoyu(Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing,Kunming 650201;Development and Utilization of Food and Drug Homology Resources Engineering Center Ministry of Education,Kunming 650201;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201;Pu'er University,Pu'er 665000,Yunnan;Animal Medical College,Yunnan Agricultural University,Kunming 650201)

机构地区：[1]云南省精准营养与个性化食品制造重点实验室,昆明650201 [2]食药同源资源开发与利用教育部工程研究中心,昆明650201 [3]云南农业大学食品科学技术学院,昆明650201 [4]普洱学院,云南普洱665000 [5]云南农业大学动物医学院,昆明650201

出　　处：《中国食品学报》2025年第1期39-50,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：云南省省市一体化专项(202302AN360002);云南省高层次科技人才及创新团队选拔专项(202305AS350025);云南省“兴滇英才支持计划”青年人才项目(2022-0255)。

摘　　要：为探究核桃膳食纤维对乳杆菌生长的潜在作用,分别添加不同质量浓度的膳食纤维(0.1~3.2 g/L)到MRS培养基中,比较其对10种乳杆菌生长的影响,以筛选出最佳的“纤维+菌种”组合进行增殖作用研究。结果表明,核桃不溶性纤维仅对植物乳植杆菌生长有促进作用,促进率最高为9.95%,对其它9种乳杆菌均有抑制作用。核桃可溶性纤维对唾液联合乳杆菌促进率最高,达44.34%,其次是植物乳植杆菌(16.14%)和干酪乳酪杆菌(11.23%)。进一步研究发现,核桃可溶性纤维可显著提高唾液联合乳杆菌的生物量、活菌数和非特异性黏附能力(P<0.05),在纤维添加量为3.2 g/L时,生物量和活菌数分别提高2.86倍和7.28倍,表面疏水性提高8.81%;显著促进其生物膜的形成(P<0.05);显著提高唾液联合乳杆菌对糖源的利用,并降低发酵液的pH值(P<0.05),在纤维添加量为3.2 g/L时,发酵液的pH值最低,为3.74。此外,核桃可溶性纤维还可显著升高唾液联合乳杆菌β-半乳糖苷酶和乳酸脱氢酶的活性(P<0.05)。结论:核桃可溶性纤维可通过提高唾液联合乳杆菌的β-半乳糖苷酶和乳酸脱氢酶的活性来促进增殖,且有利于促进其黏附性与生物膜的形成。本研究结果为核桃膳食纤维作为新型益生元提供了理论参考,为其综合开发利用提供了新思路。To investigate the potential of walnut dietary fiber to promote the growth of Lactobacilli,various mass concentrations of dietary fiber(0.1 to 3.2 g/L)were added to MRS medium.The effects of walnut dietary fiber on the growth of ten strains of Lactobacilli were compared,and the optimal'fiber+strain'combination was selected based on promotion rate to evaluate the proliferation effects.The results showed that walnut insoluble fiber only promoted the growth of Lactiplantibacillus plantarum by up to 9.95%,while inhibiting the growth of the other nine strains.In contrast,walnut soluble fiber exhibited the highest promotion rate for Ligilactobacillus salivarius at 44.34%,followed by Lactiplantibacillus plantarum(16.14%)and Lacticaseibacillus casei(11.23%).The effect of walnut soluble fiber on the proliferation of Ligilactobacillus salivarius were as follows:walnut soluble fiber significantly increased the biomass,viable count,non-specific adhesion ability(P<0.05).At a fiber concentration of 3.2 g/L,the biomass and viable count increased by 2.86 times and 7.28 times,respectively,and the surface hydrophobicity increased by 8.81%.Additionally,it significantly promoted the formation of biofilm(P<0.05),increased sugar utilization,and reduced the pH value of the fermentation broth(P<0.05).At 3.2 g/L fiber addition,the pH value of the fermentation broth reached its lowest point at 3.74.Furthermore,walnut soluble fiber significantly increased the activities ofβ-galactosidase and lactate dehydrogenase in Ligilactobacillus salivarius(P<0.05).Conclusion:walnut soluble fiber promotes the proliferation of Ligilactobacillus salivarius by increasing the activities ofβ-galactosidase and lactate dehydrogenase,and is beneficial to promoting its adhesion and biofilm formation.These findings provide a theoretical reference for the use of walnut dietary fiber as a new type of prebiotics,and offer new ideas for its comprehensive development and utilization.

关 键 词：核桃膳食纤维 乳杆菌 增殖作用 

分 类 号：TS201.3[轻工技术与工程—食品科学]