大豆种皮多糖对副干酪乳杆菌6244生长及肠道黏附性的影响  

Effects of Soybean Hull Polysaccharides on the Growth and Adhesion of Lactobacillus paracasei 6244

作　　者：宋虹 陈晨 张尊琴 杨立娜 张亚茹 张银旭 刘贺 SONG Hong;CHEN Chen;ZHANG Zunqin;YANG Lina;ZHANG Yaru;ZHANG Yinxu;LIU He(College of Food Science and Technology,Bohai University,Jinzhou 121013,Liaoning;Fuxin Institute for Drug Control,Fuxin 123007,Liaoning;Department of General Surgery,The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,Liaoning)

机构地区：[1]渤海大学食品科学与工程学院,辽宁锦州121013 [2]阜新市药品检验所,辽宁阜新123007 [3]锦州医科大学第一附属医院普通外科,辽宁锦州121001

出　　处：《中国食品学报》2025年第1期142-150,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：辽宁省自然科学基金面上项目(2023-MS-293);辽宁省“兴辽英才计划”项目(XLYC2002039);辽宁省“百千万人才工程”人选科技活动支持项目(LNBQW2019B0089)。

摘　　要：目的:探究两种大豆种皮多糖对益生菌生长及肠道黏附性的影响。方法:通过对OD600nm吸光度、生长代时及pH值的测定,分析大豆种皮多糖对副干酪乳杆菌6244增殖能力的影响。构建小鼠肠组织益生菌黏附模型,从黏附能力(表面疏水性、自聚力和共聚力)及肠道微环境的变化等研究大豆种皮多糖对副干酪乳杆菌6244生长及肠道黏附能力的影响。结果表明:0.5 mg/mL C-ASP和HW-ASP培养显著加快了副干酪乳杆菌6244的生长速度,生长代时分别缩短至4.08 h和4.69 h,然而对副干酪乳杆菌62445的产酸能力影响不显著。C-ASP显著提高了副干酪乳杆菌6244表面疏水力及共聚力,促进胞外多糖、表层蛋白等黏附素的分泌,增加了副干酪乳杆菌6244在不同肠段的定植能力,尤其在空肠和回肠肠段其黏附率均提高了32%以上。结论:大豆种皮多糖促进了肠道内益生菌的增殖和黏附,改善了肠道微环境,有利于肠道益生菌发挥活性作用。Purpose:To explore the effect of soybean hull polysaccharides on the growth and adhesion of probiotics.Methods:The absorbance of OD600nm,growth time and pH value were explored to analyze the proliferation capacity of Lactobacillus paracasei 6244.A mouse intestinal probiotic adhesion model was established to study the effects of soybean hull polysaccharides on the adhesion capacity of Lactobacillus paracasei 6244 from measuring the changes of auto-aggregation,co-aggregation,hydrophobicity and intestinal microenvironment.The results showed that 0.5 mg/mL C-ASP and HW-ASP significantly accelerated the growth rate of Lactobacillus paracasei 6244,shortened the growth time to 4.08 h and 4.69 h,respectively,but had no significant effect on the acid-producing capacity of Lactobacillus paracei 62445.In addition,C-ASP can significantly improve the surface hydrophobicity and co-aggregation of Lactobacillus paracasei 6244,promoted the secretion of exopolysaccharides,surface proteins and other adhesins and intestinal mucus,and increased the colonization of Lactobacillus paracasei 6244 in different intestinal tissue,especially in the jejunum and ileum segments,the adhesion rate of Lactobacillus paracasei 6244 increased by more than 32%.Conclusion:Soybean hull polysaccharide can promote the proliferation and adhesion of intestinal probiotics,which is conducive to the active role of intestinal probiotics and the improvement of intestinal microenvironment.

关 键 词：大豆种皮多糖 副干酪乳杆菌 增殖 黏附 

分 类 号：TS201.3[轻工技术与工程—食品科学]