女贞子多糖的提取及其抗氧化活性研究  

Extraction and Antioxidant Properties of Polysaccharides from Ligustrum lucidum

作　　者：刘艳红 纪红缘 柳如梦 张静茹 王心如 颛孙娇娇 LIU Yanhong;JI Hongyuan;LIU Rumeng;ZHANG Jingru;WANG Xinru;ZHUANSUN Jiaojiao(Biology and Food Engineering School,Fuyang University of Technology,Fuyang 236000;Biology and Food Engineering School,Fuyang Normal University,Fuyang 236000;College of Information Engineering,Fuyang Normal University,Fuyang 236000)

机构地区：[1]阜阳理工学院生物与食品工程学院,阜阳236000 [2]阜阳师范大学生物与食品工程学院,阜阳236000 [3]阜阳师范大学信息工程学院,阜阳236000

出　　处：《食品工业》2025年第1期12-16,共5页The Food Industry

基　　金：安徽省高等学校自然科学重点项目(2023AH052854,2023AH052855);安徽省优秀青年培育重点项目(YQZD2024101);安徽省质量工程项目(2023kcszsf233,22023xsxx408);国家级、省级大学生创新创业计划项目(202413619004,s202313619013,202213619003);阜阳师范大学校级项目(FXG2021ZY02,2021FXJY05,2022XGJYZD01,2022XGJX04);阜阳地方农产品食品工艺开发科研创新团队(FX2020KCT02)资助。

摘　　要：为了探究女贞子多糖的抗氧化抑菌特性及其作用机理、拓宽女贞子的开发应用渠道,采用超声辅助有机溶剂法提取女贞子多糖。通过研究女贞子多糖对·OH、O_(2)^(-)·、ABTS^(+)·的清除能力和铁还原力,探究女贞子多糖的抗氧化特性。结果表明,超声辅助有机溶剂法提取女贞子多糖的最佳条件是超声功率320 W、超声时间60 min、料液比1∶30(g/mL),此时女贞子多糖的提取率最高,为4.23%。女贞子多糖具有较好的抗氧化活性,其对·OH、O_(2)^(-)·和ABTS^(+)·均具有清除作用和铁还原作用。当多糖质量浓度为2.8 mg/mL时,其对·OH的清除率为54.65%。当多糖质量浓度为4.0 mg/mL时,其对O_(2)^(-)·和ABTS^(+)·的清除率分别为17.69%和90.59%。In order to explore the antioxidative and bacteriostatic properties of Ligustrum lucidum polysaccharide and its action mechanism,the development and application channels of Ligustrum lucidum were expanded.The ultrasonic assisted organic solvent method was used to extract Ligustrum lucidum polysaccharide.The antioxidant properties of Ligustrum lucidum polysaccharide were investigated by studying its scavenging capacity and iron reduction capacity to·OH,O_(2)^(-),ABTS^(+)·.The results showed that the optimal extraction conditions were as follows:when the ultrasonic power was 320 W,the ultrasonic time was 60 min,and the ratio of solid to liquid was 1∶30(g/mL),the extraction rate of Ligustrum lucidum polysaccharide was 4.23%.The polysaccharide of Ligustrum lucidum had good antioxidant activity,and it could clear·OH,O_(2)^(-)·and ABTS^(+)·and reduce iron.When the mass concentration of polysaccharide was 2.8 mg/mL,the clearance rate of·OH was 54.65%.When the mass concentration of polysaccharide was 4.0 mg/mL,the clearance rate of O_(2)^(-)·and ABTS^(+)·was 17.69%and 90.59%,respectively.

关 键 词：女贞子多糖 超声辅助提取 多糖提取率 工艺优化 抗氧化活性 

分 类 号：R28[医药卫生—中药学]