生香酵母生物转化法合成2-苯乙醇的研究  

Research on synthesis of 2-phenylethanol by a wild-type aroma-producing yeast through biotransformation method

作　　者：王晴 毛银 赵运英[1,2] 邓禹 WANG Qing;MAO Yin;ZHAO Yunying;DENG Yu(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学,粮食发酵与食品生物制造国家工程研究中心,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与发酵工业》2025年第5期22-28,共7页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2022YFA0911800);国家自然科学基金项目(22377039);江苏省自然科学基金项目(BK20220089)。

摘　　要：2-苯乙醇是一种具有玫瑰香味的芳香族醇,被广泛应用于食品、日化和医药等领域中。该研究利用从发酵酱醪中筛选出的一株野生型生香酵母-贝酵母,通过生物转化方法探索了该生香酵母合成2-苯乙醇的能力。通过优化反应体系中葡萄糖、底物L-苯丙氨酸、无氨基酵母氮源及其他无机盐等成分构建生物转化体系,建立了适用于该生香酵母生产2-苯乙醇的转化方法。实验结果显示,该生香酵母转化48 h后2-苯乙醇的产量最高,为2.89 g/L。在此基础上,进一步优化了转化体系中碳源、氨基受体α-酮戊二酸和离子混合营养液的浓度,2-苯乙醇的浓度提高了67%。最后,通过优化转化体系的pH、温度和溶氧等条件使得2-苯乙醇的质量浓度达到了6.05 g/L,较初始转化条件提高了109%。该方法所获2-苯乙醇不仅具有天然产物的属性和安全性,且其产量和转化率高,工艺也具有周期短和成本低的特点,具有很好的应用前景。As a rose-scented aromatic alcohol,2-phenylethanol has been widely used in the fields of food,daily chemicals,and medicine.In this study,a wild-type aroma-producing yeast(Saccharomyces bayanus)selected from fermented sauce mash was utilized to explore its ability to produce 2-phenylethanol through biotransformation methods.A biotransformation method suitable for producing 2-phenylethanol by S.bayanus was established by optimizing the ingredients of glucose,substrate L-phenylalanine,yeast nitrogen base,and other inorganic salts in the reaction system.It was shown that the highest titer of 2-phenylethanol was obtained after 48 h of transformation.On this basis,by further optimizing the concentrations of carbon source,amino acceptor ofα-ketoglutaric acid as well as the mixed ionic nutrient solution,the titer of 2-phenylethanol was reached to 4.83 g/L,an increase of 67.13%compared to that of the initial conversion conditions.Finally,by optimizing the pH,temperature,and dissolved oxygen of the conversion system,the concentration of 2-phenylethanol reached 6.05 g/L,an increase of 109.34%compared to that of the initial conversion conditions.The 2-phenylethanol obtained by this method not only has good properties and the safety of natural products,but also has a high yield and conversion rate.In addition,the process also has the characteristics of short cycle and low cost.Therefore,this biological transformation method has good application prospects.

关 键 词：生香酵母 生物转化 2-苯乙醇 L-苯丙氨酸 

分 类 号：TQ243.4[化学工程—有机化工] TQ923[轻工技术与工程—发酵工程]