紫黑香禾糯米糠黄酮代谢组学与抗氧化活性分析  

Metabolomics and Antioxidant Capacity Analysis of Flavonoids in the Bran of Purple-Black Kam Sweet Rice(Oryza sativa L.var.glutinosa Matsum)

作　　者：汪燕 陈旭玲 赵春霞 陈其皎 孟子烨 王继玥[2] 杨丽 赵顺江 梁成刚 WANG Yan;CHEN Xuling;ZHAO Chunxia;CHEN Qijiao;MENG Ziye;WANG Jiyue;YANG Li;ZHAO Shunjiang;LIANG Chenggang(School of Life Sciences,Guizhou Normal University,Guiyang 550025,China;Guiyang University,Guiyang 550005,China)

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550025 [2]贵阳学院,贵州贵阳550005

出　　处：《食品科学》2025年第6期192-200,共9页Food Science

基　　金：贵州省高层次创新型千层次人才项目(111-0920008);贵州师范大学拔尖人才振兴计划项目(黔师拔尖[2023]010);贵州师范大学学术新苗基金项目(黔师新苗[2021]A17号;黔师新苗[2022]A13号)。

摘　　要：对不同色泽紫黑香禾糯米糠进行黄酮代谢组学研究,分析其抗氧化能力,并探讨代谢物差异及其与抗氧化活性间的关系。结果发现,在米糠中共鉴定到10类139种黄酮类代谢物,紫黑香禾糯牙定纱、苟嫩当和白香禾糯苟阳弄分别含7、2种和7种特有代谢物。牙定纱vs苟阳弄、苟嫩当vs苟阳弄、牙定纱vs苟嫩当分别存在111、104种和96种差异代谢物。不同色泽米糠中花青素、原花青素、二氢黄酮醇和查耳酮类代谢物含量差异显著。深紫色牙定纱总黄酮含量最高,清除1,1-二苯基-2-三硝基苯肼自由基和2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基能力最强,浅紫色苟嫩当次之,表明种皮颜色越深,黄酮含量越高,抗氧化能力越强。共72个黄酮类代谢物与抗氧化能力呈显著或极显著正相关。本研究结果可为紫黑香禾糯米糠的加工、利用以及黄酮代谢研究提供科学参考。Metabolomic analysis and antioxidant activity of flavonoids were investigated in the bran of deep purple-black(cv.Yadingsha),light purple-black(cv.Gounendang)and white(cv.Gouyangnong)Kam Sweet Rice(KSR).Differential flavonoid metabolites between the three varieties of KSR were determined and the correlation between flavonoid metabolites and antioxidant activity was analyzed.The results showed that a total of 139 flavonoids,belonging to 10 different classes,were identified in the rice bran.Seven,two and seven unique metabolites were found in Yadingsha,Gounendang,and Gouyangnong,respectively.Additionally,111,104,and 96 differential metabolites were found in Yadingsha versus Gouyangnong,Gounendang versus Gouyangnong,and Yadingsha versus Gounendang,respectively.Significant differences were observed in the contents of anthocyanins,proanthocyanidins,dihydroflavonols and chalcones among rice brans of different kernel colors.Yadingsha exhibited the highest total flavonoid content and demonstrated the strongest scavenging activity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)cation radical,followed by Gounendang,suggesting a positive correlation between kernel color depth and flavonoid content as well as antioxidant capacity.A total of 72 flavonoid metabolites showed significant or highly significant positive correlations with antioxidant capacity.This study provides a scientific basis for the processing and utilization of purple-black KSR bran and further research on its flavonoid metabolism.

关 键 词：紫黑香禾糯 米糠 黄酮 代谢组 抗氧化活性 

分 类 号：S511.23[农业科学—作物学] S38S326